目的:探讨西红花苷对阿尔茨海默病(alzheimer’s disease,AD)大鼠空间学习记忆的影响及机制。方法:72只SD大鼠随机分为正常对照组、模型对照组、白藜芦醇组(40mg/kg)和西红花苷低(20mg/kg)、中(40mg/kg)、高(80mg/kg)剂量组。侧脑室微量注射5μl Aβ25-35建立AD大鼠模型,从次日开始每天于固定时间给药,正常对照组和模型对照组注入等量的生理盐水,剂量均为10μl,连续注射14d。利用Morris水迷宫对大鼠进行行为学试验,检测血清活性氧基团(ROS)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,免疫组化实验检测海马CA3区和前额叶皮层GAP-43蛋白的表达。结果:Morris水迷宫实验中,模型对照组大鼠逃避水下平台的潜伏期时间较正常组显著延长;白藜芦醇组和西红花苷各剂量组寻找水下平台的潜伏期时间从d2起,较模型对照组均显著缩短;西红花苷40、80mg/kg组潜伏期时间均显著短于20mg/kg组,但与白藜芦醇组之间在各实验日的潜伏期均未见有无显著性统计学差异。血清氧化应激因子的检测,白藜芦醇组和西红花苷各剂量组大鼠血清ROS、MDA含量均显著性高于正常组,低于模型对照组,SOD的变化与此相反。免疫组化实验中,白藜芦醇组和西红花苷各剂量组大鼠海马CA3区和前额叶皮层GAP-43的表达显著性低于正常组,高于模型对照组。结论:西红花苷可能通过抗氧化和增加海马及前额叶皮层GAP-43的表达对AD大鼠空间辨别性学习记忆能力有一定的改善作用。 Objective: To investigate the effect and mechanism of crocin on spatial learning and memory in Alzheimer’s disease (AD) rats. Methods: Seventy two SD rats were randomly divided into normal control group, model control group, resveratrol group (40mg / kg) and crocetin low (20mg / kg) / kg) dose group. The rat model of AD was established by microinjection of 5μl of Aβ25-35 into the lateral ventricle. The rats in the normal control group and the model control group were injected with the same amount of normal saline. The dose was 10μl and injected continuously for 14 days. The Morris water maze was used to conduct behavioral tests in rats. Serum levels of reactive oxygen species (ROS), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Immunohistochemical staining was used to detect the hippocampal CA3 region and forehead Expression of GAP-43 protein in leaf cortex. Results: In the Morris water maze test, the latent period of escape from the underwater platform in the model control group was significantly longer than that in the normal group. The latency period of the resveratrol group and the crocetin searching submarine platform started from d2, Compared with the model control group were significantly shortened; crocin 40,80mg / kg group latency was significantly shorter than 20mg / kg group, but with the resveratrol group in the incubation period of each experiment were no significant Sexual statistics differences. Serum oxidative stress factor detection, resveratrol group and crocidin dose of serum ROS and MDA levels were significantly higher than the normal group, lower than the model control group, SOD changes in the opposite. In immunohistochemistry, the expression of GAP-43 in hippocampal CA3 area and prefrontal cortex in resveratrol group and crocinil group was significantly lower than that in normal group and higher than that in model control group. CONCLUSION: Crocetin may play an important role in improving spatial learning and memory abilities of rats with AD through antioxidation and increase of GAP-43 expression in hippocampus and prefrontal cortex.