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利用荧光发射光谱技术及荧光诱导动力学技术 ,研究了不同基因型大豆 :高产大豆黑农40和黑农41及低产大豆黑农37的光能吸收、传递和转化等特性。结果表明 ,高产大豆的叶绿素(Chl)和类胡萝卜素(Car)的含量均高于黑农37 ,但不同的品种差异幅度有所不同。吸收光谱表明高产大豆叶绿体对光能有更强的吸收能力 ;Mg2 + 对高产大豆叶绿体PSI和PSII之间激发能分配的调节能力均高于低产品种 ;高产大豆叶片PSII原初光能转化效率(Fv/Fm)和PSII活性(Fv/Fo) ,光化学猝灭系数(qP)及PSII总的光化学量子产量均高于低产品种相应的值 ;而高产品种的非光化学猝灭系数(qN)低于低产品种。实验说明 ,高产大豆叶片具有与产量潜力相关的较高光能吸收和原初转化能力。
Using fluorescence emission spectroscopy and fluorescence-induced kinetic techniques, the characteristics of light energy absorption, transfer and transformation of different genotype soybean: high-yielding soybean Heinong 40 and Heinong 41 and low-yielding soybean Heinong 37 were studied. The results showed that the contents of chlorophyll (Chl) and carotenoids in high yielding soybean were higher than those in Heinong 37, but the differences of different varieties were different. Absorption spectra showed that high yield soybean chloroplasts had a stronger ability to absorb light energy. Mg2 + had more regulation ability on excitation energy distribution between PSI and PSII of high yield soybean than that of low yielding varieties. The primary light energy conversion efficiency Fv / Fm), PSII activity (Fv / Fo), photochemical quenching coefficient (qP) and total photochemical quantum yield of PSII were all higher than those of low yielding varieties. However, the non-photochemical quenching coefficient (qN) In low-yield varieties. Experiments show that high yield soybean leaves have higher light energy absorption and initial conversion ability related to yield potential.