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目的:探讨敲除miR-150基因对铅暴露小鼠神经行为和学习功能的影响。方法:SPF级C57BL/6小鼠和miR-150基因敲除小鼠各24只,分别随机分为两组即对照组和染毒组,每组12只。采用自由饮水方式进行染毒,对照组饮用2g/L醋酸钠,染毒组饮用0.5g/L醋酸铅,持续8周,小鼠醋酸铅的暴露剂量为80mg·kg-1。染毒结束后进行热板仪、矿场实验、Y型电迷宫等神经行为学和学习记忆的测试。结果:在感觉神经功能的损伤方面,K/O组和WT组之间没有差异(P>0.05);在焦虑、空间识别能力、空间探索能力以及学习记忆能力方面,KO组和WT组有显著差异(P<0.05)反应时间明显延长。结论:miR-150基因对铅暴露小鼠感觉神经功能无明显影响,miR-150基因可抑制铅暴露小鼠产生的焦虑以及空间识别、空间探索和学习记忆功能的下降。
Objective: To investigate the effect of knocking out miR-150 gene on the neurobehavioral and learning functions of lead-exposed mice. Methods: Twenty-four SPF C57BL / 6 mice and 24 miR-150 knockout mice were randomly divided into two groups: control group and exposure group, with 12 mice in each group. The rats in the control group were given 2g / L sodium acetate. The exposure group consumed 0.5g / L lead acetate for 8 weeks. The exposure dose of the mouse lead acetate was 80mg · kg-1. After the end of the exposure to hot plate, mine experiments, Y-type electric maze and other neurological behavior and learning and memory test. Results: There was no significant difference in sensory nerve function between KO group and WT group (P> 0.05). There was significant difference between KO group and WT group in anxiety, spatial recognition ability, space exploration ability and learning and memory ability Difference (P <0.05) reaction time was significantly prolonged. Conclusion: The miR-150 gene has no significant effect on the sensory nerve function in lead-exposed mice. MiR-150 gene can inhibit anxiety and spatial recognition, space exploration and learning and memory function of lead-exposed mice.