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目的探讨江苏省汉族人群蛋白激酶Cη(PKCη)基因A1427C单核苷酸多态性与动脉粥样硬化性脑梗死的关系。方法选择120例动脉粥样硬化性脑梗死患者,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测PKCη基因A1427C多态性、采用颈动脉彩色多普勒超声检测颈总动脉内膜-中层厚度,并与对照组100例年龄、性别相匹配的非神经科住院患者和健康体检者比较。将所有研究对象的脑梗死危险因素做多元Logistic回归分析。结果脑梗死组中C等位基因频率(40.8%)及CC+AC基因型频率(65.0%)明显高于对照组(29.5%和49.0%),P<0.05;携带C等位基因的个体与非携带C等位基因的个体比较,患脑梗死的OR值为1.649,(95%CI:1.108~2.456);与AA基因型比较,CC+AC基因型患脑梗死的OR值为1.933,(95%CI:1.123~3.326)。脑梗死组颈总动脉内膜-中层厚度与对照组比较差异有统计学意义(P<0.05)。Logistic回归分析显示,吸烟、高脂血症和C等位基因患脑梗死的OR值分别为2.102、2.004及1.857。结论PKCη基因A1427C多态性可能与动脉粥样硬化性脑梗死的发生相关,C等位基因可能是脑梗死发生的独立危险因素。
Objective To investigate the association between A1427C single nucleotide polymorphism of protein kinase Cη (PKCη) gene and atherothrombotic cerebral infarction in Han nationality in Jiangsu Province. Methods One hundred and twenty patients with atherosclerotic cerebral infarction were selected and the polymorphism of PKCη gene A1427C was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Carotid artery color Doppler ultrasound Intima-media thickness of the common carotid artery were compared with 100 non-neurological inpatients and healthy subjects who were matched in age and sex in the control group. Multivariate Logistic regression analysis was performed on risk factors of cerebral infarction in all subjects. Results The frequencies of C allele (40.8%) and CC + AC genotype (65.0%) in cerebral infarction group were significantly higher than those in control group (29.5% and 49.0%, P <0.05) Compared with AA genotypes, the OR of cerebral infarction with CC + AC genotype was 1.933 (95% CI: 1.108-2.456), compared with those without C allele 95% CI: 1.123 ~ 3.326). Intima - media thickness of common carotid arteries in cerebral infarction group was significantly different from that in control group (P <0.05). Logistic regression analysis showed that OR of smoking, hyperlipidemia and C allele were 2.102, 2.004 and 1.857, respectively. Conclusion The A1427C polymorphism of PKCη gene may be related to the occurrence of atherosclerotic cerebral infarction. C allele may be an independent risk factor for cerebral infarction.