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目的了解Kaposi肉瘤组织中人类疱疹病毒8型(HHV-8)K15等位基因型分布情况,并初步探讨Kaposi肉瘤不同临床分型及临床表现与不同HHV-8 K15等位基因型的相关性。方法采用酚-氯仿-异戊醇法对收集的27例Kaposi肉瘤石蜡包埋组织标本进行病毒DNA抽提,并使用巢式PCR扩增K15基因片段,然后测序并确定其等位基因型。结果27例Kaposi肉瘤中有22例HHV-8感染为阳性,阳性率为81.48%,其中4例艾滋病-Kaposi肉瘤患者HHV-8感染均为阳性,感染率100%;在分析的22例HHV-8病毒株中,20例为P型,2例为M型;4例艾滋病-Kaposi肉瘤患者感染的均为P型HHV- 8,2例M型感染者均为经典型Kaposi肉瘤患者。结论Kaposi肉瘤组织内HHV-8的K15等位基因型主要是P型,也存在部分M型HHV-8感染者。4例艾滋病-Kaposi肉瘤患者感染的HHV-8均为P型。
Objective To investigate the distribution of human herpesvirus type 8 (HHV-8) K15 alleles in Kaposi sarcoma and to investigate the relationship between different clinical types and clinical manifestations of Kaposi sarcoma and different HHV-8 K15 alleles. Methods 27 samples of Kaposi ’s sarcoma paraffin - embedded tissue samples were extracted with DNA by phenol - chloroform - isoamyl alcohol method. K15 gene fragment was amplified by nested PCR and sequenced to determine its allele. Results Of 27 Kaposi’s sarcomas, 22 were positive for HHV-8 infection and the positive rate was 81.48%. Among the 4 cases, HHV-8 infection was positive in all 4 cases of AIDS-Kaposi’s sarcoma, and the infection rate was 100%. Among the 22 HHV- Among the 8 strains, 20 were P-type and 2 were M-type. Four cases of AIDS-Kaposi’s sarcoma were all P-type HHV-8 and two of M-type were all Kaposi’s sarcoma. Conclusion The K15 allele of HHV-8 in Kaposi sarcoma is predominantly P-type and some M-type HHV-8 infected. Four cases of AIDS-Kaposi sarcoma patients infected with HHV-8 are P-type.