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2008年对印度北方邦东部Gorakhpur、Kushinagar与Maharajganj等3个地区的西葫芦种植区进行调查,发现西葫芦叶片呈现花叶斑点状、卷曲,植株黄化萎缩。以菜豆金色花叶病毒组外壳蛋白基因特异引物AV1-F、AV1-R对从不同地区收集的西葫芦病斑叶片样品进行PCR分析,获得约800 bp的扩增产物。对从Gorakhpur地区获得的分离物的扩增产物进行克隆和序列分析,测序结果提交GenBank核酸序列数据库;通过核酸BLAST比对和系统发育分析,该分离物与帕兰波番茄曲叶病毒株的同源性最高,达95%,两者遗传关系最近,认为此病毒分离物是帕兰波番茄曲叶病毒株。
In 2008, the investigation was carried out on the zucchini planting areas in Gorakhpur, Kushinagar and Maharajganj in the eastern part of Uttar Pradesh. The zucchini leaves showed patchy spots, curly leaves and atrophy of plants. A total of 800 bp PCR products were obtained from the leaves of cucurbit [cucurbitaceous patulin (Zucchini) blight collected from different regions using AV1-F, AV1-R, a coat protein gene specific primer for golden bean mosaic virus. The amplified products of isolates obtained from Gorakhpur region were cloned and sequenced. The sequencing results were submitted to the GenBank database. Through BLAST analysis and phylogenetic analysis, The highest source, up to 95%, the genetic relationship between the two recently considered that the virus isolate is the Palembo tomato leaf curl strain.