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建立用高效液相色谱法测定醋酸曲安奈德硫软膏10%中醋酸曲安奈德的含量的方法。采用高效液相色谱法,色谱柱为Diamonsil C18(250mm×4.6mm,粒直径5μm);流动相为甲醇-水(80∶20);流速为1.0ml/min;柱温:25℃;检测波长为240nm。结果表明:醋酸曲安奈德在39.48~197.40μg/ml范围内,线性关系良好,r=0.9999,回归方程为A=37.003c+41.428;稳定性试验的RSD为0.6%(n=6),精密度试验的RSD为0.14%(n=6),方法重复性的RSD为1.06%(n=6),醋酸曲安奈德的平均加样回收率为98.3%,RSD为0.7%(n=6)。在本色谱条件及样品处理方法下,可以准确测出醋酸曲安奈德硫软膏10%中醋酸曲安奈德的含量。
A method for the determination of triamcinolone acetonide in 10% triamcinolone acetonide cream by HPLC was established. The column was Diamonsil C18 (250mm × 4.6mm, particle size 5μm) with mobile phase of methanol-water (80:20), the flow rate was 1.0ml / min, the column temperature was 25 ℃, the detection wavelength 240nm. The results showed that triamcinolone acetonide showed a good linearity in the range of 39.48 ~ 197.40μg / ml, r = 0.9999, the regression equation was A = 37.003c + 41.428, RSD of stability test was 0.6% (n = 6) The RSD of the test was 0.14% (n = 6), the repeatability of the method was 1.06% (n = 6), the average recovery of triamcinolone acetonide was 98.3% and the RSD was 0.7% . Under the conditions of the chromatographic conditions and sample processing, the content of triamcinolone acetonide in 10% of triamcinolone acetonide acetate ointment can be accurately detected.