植物的成分作为化学致突变物质许多实验室已积极地进行了检测和评价,表明许多成分是强烈地致突变的,而且其中某些对高等动物是致癌的(Kada,1977)。另一方面,Kada 等(1978)和 Morifa 等(1978)报告了姜和洋白菜的汁中含有对抗蛋白质和氨基酸热解时色氨酸热解产物致突性的抗致突变因子。作者发现姜的汁中含有促进和抑制细菌突变作用的两种因子。作者以2(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺(简称 AF2)和 N-甲基-N’-硝基-N-亚硝基胍(简称 NTG)为阳性致突变物。以有遗传标志 uvrB(DNA 紫外线损伤修复有缺陷的)和 argFam(需要精氨酸的琥珀突变)的大肠埃希菌 B/r 的 Hs30菌株作为致突作用的指示菌株。并用 H/r30菌株(uvr~+arg Fam)作为 Hs30的参考。将 Hs30菌株接种于按 Komdo 和 Ichikawa-Ryo(1973)的方法制备的选择培养基 E 上,37℃培养48小时,测 The composition of plants as chemical mutagenic substances Many laboratories have actively tested and evaluated that many components are strongly mutagenic and some of them are carcinogenic to higher animals (Kada, 1977). On the other hand, Kada et al. (1978) and Morifa et al. (1978) reported on the antimutagenicity of tryptophan pyrolysis products against pyrolysis of proteins and amino acids in ginger and cabbage juices. The authors found that ginger juice contains two factors that promote and inhibit bacterial mutations. Authors 2(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF2 for short) and N-methyl-N’-nitro-N-nitrosoguanidine (NTG for short) ) is a positive mutagen. The Hs30 strain of Escherichia coli B/r, which has the genetic markers uvrB (defective for DNA damage by ultraviolet damage repair) and argFam (amber mutation requiring arginine), was used as an indicator strain for the onset of neuritogenesis. The H/r30 strain (uvr~+arg Fam) was used as a reference for Hs30. The Hs30 strain was inoculated on selection medium E prepared by the method of Komdo and Ichikawa-Ryo (1973) and cultured at 37°C for 48 hours.