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本研究探讨以阳离子没食子酸丙酯(CPG)为激活剂的血小板聚集试验用于单采血小板捐献者筛查的可行性,并调查血小板献血者血小板功能缺陷的发生率。测定不同浓度CPG诱导的健康献血者的血小板聚集率,以确定CPG的最适应用浓度;检测30名志愿者服用阿司匹林前和服药24小时后的血小板聚集率,以确定血小板功能不良的筛查界点值;检测483例血小板捐献者的CPG诱导的血小板聚集率,并对聚集功能不良者进行活化血浆凝固时间(APCT)测定。结果表明:血小板聚集率随CPG浓度的增加而升高,当CPG浓度达200μmol/L时,血小板聚集率达最高;服用阿司匹林24小时后的血小板聚集率与服药前相比,均表现明显减低(P<0.001),但以CPG诱导180秒时的血小板聚集率减低最显著。血小板功能不良的筛查界点值确定为CPG诱导180秒时的血小板聚集率小于20%;在483例血小板捐献者中,检出25例有血小板聚集功能不良,其中有11例表现为血小板促凝血活性减低。结论:CPG诱导的血小板聚集试验能有效的检出血小板功能不良者,适用于血小板捐献者血小板功能的筛选;在血小板献血者中,血小板功能缺陷者的检出率大约为5%。
This study investigated the feasibility of platelet aggregation assay using cation gallate gallate (CPG) as an activator for platelet donor screening and investigated the incidence of platelet function defect in platelet donors. Determine the platelet aggregation rate of healthy blood donors induced by different concentrations of CPG to determine the most suitable concentration of CPG; detect the platelet aggregation rate of 30 volunteers before taking aspirin and after 24 hours to determine the platelet dysfunction The value of platelet aggregation was detected in 483 platelet donors and activated plasma coagulation time (APCT) was measured in patients with poorly aggregated function. The results showed that the platelet aggregation rate increased with the increase of CPG concentration. When the concentration of CPG reached 200 μmol/L, the platelet aggregation rate was the highest; the platelet aggregation rate after 24 hours of aspirin use was significantly reduced compared with that before administration ( P<0.001), but the most significant decrease in platelet aggregation rate was 180 seconds after CPG induction. The platelet aggregation threshold for platelet dysfunction was determined to be less than 20% platelet aggregation at 180 seconds of CPG induction; of the 483 platelet donors, platelet aggregation dysfunction was detected in 25 cases, 11 of which were platelet-promoting. Coagulation activity is reduced. Conclusion: The platelet aggregation test induced by CPG can effectively detect platelet dysfunction and is suitable for the screening of platelet function in platelet donors. Among platelet donors, the detection rate of platelet dysfunction is approximately 5%.