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在离体条件下,以我国主栽香蕉品种‘巴西蕉’(Musa AAA Cavendish)的多芽体和不定芽为材料,采用EMS和NaN3对其进行诱变处理,以镰刀菌酸毒素为选择压,筛选抗镰刀菌酸的材料。结果表明,EMS和NaN3诱变处理多芽体适宜的浓度和时间组合分别为1.6%+3h和2g/L+3h,NaN3诱变处理不定芽适宜的浓度和时间组合为2g/L+1h。镰刀菌酸毒素筛选多芽体和不定芽适宜浓度范围分别为36～45mg/L和10～20mg/L,筛选获得的多芽体和不定芽多次继代培养后仍保持对镰刀菌酸的抗性。本研究可为香蕉抗枯萎病育种提供有价值的材料和技术参考。
Under in vitro conditions, the multi-buds and adventitious buds of Musa AAA Cavendish, a main banana cultivar in our country, were mutagenized with EMS and NaN3, with Fusarium acid toxin selective , Screening anti-Fusaric acid material. The results showed that the suitable concentration and time combination of mutagenesis of EMS and NaN3 were 1.6% +3 h and 2 g / L + 3 h, respectively. The optimum concentration and time of NaN3 mutagenesis for adventitious buds was 2 g / L + 1 h. Fusarium acid toxins screening of polygamy and adventitious buds suitable concentration range of 36 ~ 45mg / L and 10 ~ 20mg / L, screening of buds and adventitious buds after repeated subcultures remain Fusaric acid Resistant. This study can provide valuable materials and technical reference for the breeding of banana wilt resistance.