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目的探讨p53和c-erbB-2的表达与胃癌浸润的关系。方法收集139例胃癌手术切除标本,包括黏膜内癌23例、浸润至黏膜下层31例、浸润至肌层47例、浸润至浆膜或浆膜外38例,切片采用免疫组化SP法染色。采用多分类有序反应变量logistic回归进行统计分析。结果不同浸润深度的胃癌中p53的阳性率比较,黏膜内癌组和浸润至黏膜下层组与浸润至浆膜层或浆膜外组存在显著差异。而各组c-erbB-2的表达阳性率比较差异均有统计学意义(P<0.01)。p53的表达强度在不同的浸润深度无统计学意义(P=0.149>0.05)。c-erbB-2差别明显,在胃癌浸润至浆膜或浆膜外时,其表达强度最高(P=0.002<0.01)。p53和c-erbB-2的阳性表达对胃癌的浸润均有促进作用(P值分别为0.036、0.000)。p53和c-erbB-2的不同表达强度中,p53Ⅲ、c-erbB-2Ⅰ、c-erbB-2Ⅱ和c-erbB-2Ⅲ对胃癌浸润深度均有影响(P值分别为0.001、0.01、0.000、0.000)。结论抑癌基因p53突变可能与胃癌的起源有关;胃癌的浸润主要与癌基因c-erbB-2的激活有关。
Objective To investigate the relationship between p53 and c-erbB-2 expression and gastric cancer infiltration. Methods Totally 139 gastric cancer specimens were collected, including 23 cases of intramucosal carcinoma, 31 cases infiltrating into the submucosa, 47 cases infiltrating into the muscular layer, 38 cases infiltrating into the serosa or serosa, and the sections were stained with immunohistochemical SP method. Statistical analysis was carried out using logistic regression with multi-classification and orderly response variables. Results The positive rate of p53 in gastric cancer with different depth of invasion was significantly different between the intramucosal cancer group and the submucosal invasion group and the subserosa or serosa group. The positive rates of c-erbB-2 in each group were significantly different (P <0.01). The expression of p53 in different depth of invasion was not statistically significant (P = 0.149> 0.05). The difference of c-erbB-2 was significant. The expression of c-erbB-2 was the highest when the gastric cancer infiltrated into the serosa or serosa (P = 0.002 <0.01). The positive expression of p53 and c-erbB-2 could promote the infiltration of gastric cancer (P = 0.036,0.000, respectively). The expressions of p53Ⅲ, c-erbB-2Ⅰ, c-erbB-2Ⅱ and c-erbB-2Ⅲ were significantly different between gastric cancer and gastric cancer (P = 0.001,0.01,0.000, 0.000). Conclusion The mutation of p53 tumor suppressor gene may be related to the origin of gastric cancer. The infiltration of gastric cancer is mainly related to the activation of oncogene c-erbB-2.