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目的:探讨口腔黏膜上皮组织体外培养的影响因素,并提出相应改进措施。方法:选择重庆市职业病防治院2012年12月-2014年12月接收的24例患者为研究对象取其口腔黏膜样本,采用新洁尔灭联合硫酸庆大霉素对口腔黏膜标本进行处理,观察Dispase浓度的不同对上皮分离作用的不同影响,采用酶消化法进行分离细胞,采用无血清培养液实施原代与传代培养。结果:0.25%浓度的分离率为9.09%,而0.40%浓度的分离率为53.85%,两组比较,差异具有统计学意义(P<0.05)。结论:对人口腔黏膜上皮细胞的原代培养方法进行改进,可有效简化操作步骤,成功率高,可有效控制微生物污染。浓度越高上皮分离效果越好,且细胞增殖快。
Objective: To investigate the influencing factors of oral mucosa epithelial tissue culture in vitro and to propose corresponding improvement measures. Methods: Twenty-four patients received from Chongqing Occupational Disease Prevention and Treatment Hospital from December 2012 to December 2014 were selected as the oral mucosa samples. The oral mucosa samples were treated with benzalkonium bromide and gentamycin sulfate. The concentrations of Dispase Different effects on the role of epithelial segregation, the use of enzyme digestion method for separation of cells, the use of serum-free medium for primary and subculture. Results: The separation rate of 0.25% was 9.09%, while that of 0.40% was 53.85%. There was significant difference between the two groups (P <0.05). Conclusion: The method of primary culture of human oral mucosa epithelial cells is improved, which can effectively simplify the operation steps, with high success rate and effective control of microbial contamination. The higher the concentration, the better the epithelial isolation and the rapid proliferation of cells.