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目的 研究FOLP2 3、TPOX及GABRB15的多态性及法医学应用价值。方法 应用聚合酶链反应 (PCR)、聚丙烯酰胺凝胶电泳分离及银染显带技术对武汉地区汉族 2 0 0例无关个体作FOLP2 3、TPOX及GABRB15位点分型调查。结果 FOLP2 3、TPOX及GABRB15位点分别检出 6、 5和 5个等位基因 ,获得汉族人群基因频率分布。三位点基因型频率分布符合Hardy -Weinberg平衡。位点杂合度分别为0 6 798、 0 5 6 5 0、 0 6 95 0 ,个人识别能力分别为 0 85 94、 0 6 975和 0 82 6 2 ,非父排除率分别为 0 45 31、0 2 745、 0 40 79。结论 FOLP2 3、TPOX及GABRB1 5位点均是高杂合度、高鉴别能力的遗传标记系统 ,具有较高的法医学应用价值
Objective To study the polymorphisms of FOLP2 3, TPOX and GABRB15 and the forensic application value. Methods Polymorphisms of FOLP2 3, TPOX and GABRB15 were detected in 200 Han unrelated individuals in Han area of Wuhan by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis and silver staining. Results The frequencies of 6, 5 and 5 alleles were detected in FOLP2 3, TPOX and GABRB15 loci, respectively. The frequency distribution of the three loci was in accordance with Hardy-Weinberg equilibrium. The heterozygosities at the loci were 0 6 798, 0 565 0 and 0 6 95 0, respectively, and the individual recognition capacities were 0 85 94, 0 6 975 and 0 82 6 2, respectively. The non-parent exclusion rates were 0 45 31 and 0 0 respectively 2 745, 0 40 79. Conclusion The FOLP2 3, TPOX and GABRB1 5 loci are both high heterozygosity and high discrimination genetic marker system with high forensic value