The maximum specific methanogenic activity (SMA) of a sludge originating from a brewery wastewater treatment plant on the degradation of glucose was investigated at various levels of sulfate on a specific loading basis. Batch experiments were conducted in serum bottles at pH 7 and 35℃.A comparison of the values indicates that the SMA of this mixed culture was increased and reached its highest level of 0.128g CH4 gas COD/(g VSS·d)when biomass was in contact with sulfate at a ratio of 1:0.114 by weight.