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目的:观察酪氨酸激酶受体RON在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者肺组织中的表达及分布,以探讨RON在人类COPD发病机制中的作用。方法:收集2013年5月至2013年12月在川北医学院附属医院心胸外科因肺癌进行手术治疗的患者,将其分为对照组(25例)和COPD组(20例),于手术切除标本中选取距肺癌病灶5cm以上的新鲜肺组织。采用免疫组化方法观察两组患者肺内RON蛋白的表达和分布情况。结果:COPD组患者肺组织中肺泡区与支气管区RON蛋白的表达较对照组均明显增强(P<0.05),而且COPD组患者支气管区的RON蛋白表达量多于肺泡区(P<0.01)结论:COPD患者肺组织中RON的合成与表达增强,其表达量与患者气流受限严重程度相关。
AIM: To investigate the expression and distribution of tyrosine kinase receptor RON in lung tissues of patients with chronic obstructive pulmonary disease (COPD), so as to investigate the role of RON in the pathogenesis of human COPD. Methods: From May 2013 to December 2013, patients undergoing cardiothoracic surgery due to lung cancer surgery in the Affiliated Hospital of North Sichuan Medical College were divided into control group (n = 25) and COPD group (n = 20) In the selection of lung cancer lesions more than 5cm of fresh lung tissue. Immunohistochemistry was used to observe the lung RON protein expression and distribution in both groups. Results: The expression of RON protein in bronchoalveolar lavage and bronchus in COPD group was significantly higher than that in control group (P <0.05), and the expression of RON protein in bronchus area of COPD group was more than that in alveolar area (P <0.01). : The synthesis and expression of RON in the lung tissue of patients with COPD is enhanced, and its expression correlates with the severity of airflow limitation in patients.