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目的 探讨儿童乙型肝炎病毒基本核心启动子 (HBVBCP)变异与肿瘤坏死因子 α(TNF α)水平的相关性及其意义。方法 采用核酸分子杂交技术检测儿童乙型肝炎病毒双位点变异 (T176 2 /A176 4 ) ,同时以酶联免疫吸附测定法检测其血清TNF α水平 ,另外 ,进行了肝功能检测 ,对结果进行统计学分析。结果 儿童慢性乙型肝炎轻、中、重度 3组HBVBCP基因区双变异检出率分别为 9.38%、2 8.5 7%、5 5 .5 6 % ;HBVBCP变异株病毒感染组肝功能损害程度较野生株病毒感染组严重 ,经统计学处理 ,差异有统计学意义 ;HBVBCP变异株病毒感染组血清TNF α水平显著高于非变异株病毒感染组患儿。结论 儿童慢性乙型肝炎HBVBCP双变异株病毒感染后 ,引起TNF α产生增多 ,使肝炎病变程度加重 ,对重症肝炎的防治及探讨重症乙型肝炎的发病机制有重要意义
Objective To investigate the relationship between the variation of hepatitis B virus basic core promoter (HBVBCP) and the level of tumor necrosis factor α (TNFα) in children and its significance. Methods Two-point mutation of hepatitis B virus (T176 2 / A176 4) in children was detected by nucleic acid hybridization and the level of serum TNFα was detected by enzyme-linked immunosorbent assay (ELISA). In addition, liver function tests were performed and the results were analyzed Statistical analysis. Results The detection rates of double mutation of HBV BCP gene in mild, moderate and severe chronic hepatitis B patients were 9.38%, 2.857% and 5.55% respectively. The degree of liver damage in HBV BCP virus group was significantly higher than that in wild type Strain of virus infection was severe, and the difference was statistically significant. The level of serum TNFαin HBVBCP virus group was significantly higher than that in non-virus group. Conclusions The infection of HBV BCP double mutant virus in children with chronic hepatitis B leads to increased production of TNFα and aggravating the severity of hepatitis. It is significant for the prevention and treatment of severe hepatitis and the pathogenesis of severe hepatitis B