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目的 :探讨躯体性及心理性应激状态下大鼠胃粘膜NOS变化及与胃粘膜损伤发生的关系。方法 :采用高压恒流脉冲刺激器复制胃粘膜损伤模型 ,将 96只雄性SD大鼠随机分为 3组 :对照组 (C) ,规则光组 (R)及不规则光组 (I) ,分光光度法检测NOS活性和NilsLambecht法测定胃粘膜损伤指数 ,使用兔源大鼠NOSⅠ ,NOSⅡ ,NOSⅢ多抗 ,采用免疫组化方法研究NOS的分布规律。结果 :正常情况下NOS活性平稳 ,心理性应激后 ,大鼠胃粘膜NOS活性明显升高 (P <0 .0 5) ;与对照组比较 ,R ,I组胃粘膜损伤指数均较高 ,且I组较R组变化显著 (P <0 .0 5) ;NOSⅠ ,NOSⅢ在 3组中均有表达 ,而NOSⅡ只在R ,I组中表达 ,且其表达无明显差异 (P >0 .0 5)。结论 :心理性应激程度愈高 ,胃粘膜损伤愈严重 ,胃粘膜损伤程度及NOS活性变化与心理应激程度关系密切 ,其分布变化似乎与心理应激程度无关
Objective: To investigate the relationship between gastric mucosal NOS and gastric mucosal injury in somatic and psychological stress rats. Methods: The model of gastric mucosa injury was replicated by high-voltage constant current pulse stimulator. 96 male SD rats were randomly divided into three groups: control group (C), regular light group (R) and irregular light group (I) The activity of NOS was detected by spectrophotometry and the index of gastric mucosal injury was determined by Nils Lambecht method. The distribution of NOS was studied by immunohistochemistry using rabbit polyclonal anti-NOSⅠ, NOSⅡ and NOSⅢ antibodies. Results: The activity of NOS was stable and the NOS activity of gastric mucosa was significantly increased after psychological stress (P <0.05). Compared with the control group, gastric mucosal injury index of R and I group were higher, (P <0.05). The expression of NOSⅠand NOSⅢ in all three groups was higher than that in group R, while NOSⅡwas only expressed in R and I groups (P> 0.05). 0 5). Conclusion: The higher the degree of psychological stress is, the more serious the gastric mucosal injury is. The degree of gastric mucosal injury and the change of NOS activity are closely related to the degree of psychological stress. The distribution does not seem to be related to the degree of psychological stress