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采用９５％乙醇抽提、丙酮沉淀粗提新鲜牛心中的心磷脂；应用硅胶Ｇ高压梯度洗脱液对其进行纯化；应用硅胶Ｇ薄层层析进行了鉴定；应用修改的Ｆｉｓｋｅ－Ｓｕｂｂａｒｏｗ方法对纯化的心磷脂进行定量。结果显示硅胶Ｇ纯化的心磷脂纯度高，经薄层层析呈单一黄色斑点，定磷法定量产率为每１００ｇ新鲜牛心可获心磷脂８．２３ｍｇ。结果提示：通过硅胶Ｇ层析法可获得高纯度、高产率的心磷脂。
The cardiolipin was extracted from 95% ethanol and precipitated with acetone. The purified product was purified by silica gel G high pressure gradient elution and identified by silica gel thin layer chromatography. The modified Fiske-Subbarow method Purified cardiolipin was quantified. The results showed that silica gel G purified cardiolipin high purity thin layer chromatography showed a single yellow spot, fixed phosphorus quantitative yield per 100g fresh beef heart available lecithin 8.23mg. The results suggest that: high-purity, high yield of cardiolipin can be obtained by silica gel G chromatography.