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目的调查2007年深圳市分离自生熟食品及餐具中金黄色葡萄球菌的耐药情况及对红霉素和克林霉素的诱导耐药性。方法用国标方法分离鉴定金黄色葡萄球菌,VITEK2 Compact生化验证,用K-B法进行药敏试验,用D试验检测红霉素对克林霉素的诱导耐药性。结果从食品及餐具涂抹拭子中分离的53株金黄色葡萄球菌对青霉素G、氨苄西林及阿莫西林/棒酸表现很高的耐药性,耐药率达80%以上;对万古霉素、替考拉宁、甲氧西林、氨苄西林/舒巴坦、头孢唑啉、头孢噻吩、头孢西丁7种药物均敏感;对红霉素和克林霉素同时耐药占11.3%。D试验阳性占所检测菌株11.3%;在单一纸片红霉素耐药(中介)而克林霉素敏感的菌株中,D试验阳性即对克林霉素具有诱导耐药性为25%。结论金黄色葡萄球菌食品分离株绝大部分为MSSA,除青霉素类外耐药率较低;在红霉素耐药(中介)、克林霉素敏感菌株中克林霉素诱导耐药率为25%,开展D试验检测可给临床提供具有参考价值的数据。
Objective To investigate the drug resistance of Staphylococcus aureus isolated from raw and cooked food and tableware in Shenzhen in 2007 and the induced resistance to erythromycin and clindamycin. Methods Staphylococcus aureus was isolated and identified by the national standard method. VITEK2 Compact biochemical verification was performed. Drug susceptibility test was performed by K-B method. D-test was used to test the resistance of erythromycin to clindamycin. Results 53 strains of Staphylococcus aureus isolated from food and tableware swabs showed high resistance to penicillin G, ampicillin and amoxicillin / clavulanic acid, with a resistance rate of more than 80%. For vancomycin , Teicoplanin, methicillin, ampicillin / sulbactam, cefazolin, cefalotin, cefoxitin seven kinds of drugs are sensitive to erythromycin and clindamycin simultaneously resistant accounted for 11.3%. D test positive accounted for 11.3% of the tested strains; in a single piece of erythromycin drug-resistant (intermediary) and clindamycin sensitive strains, D test positive that clindamycin induced resistance was 25%. Conclusion Staphylococcus aureus foodstuffs isolates are mostly MSSA, with the exception of penicillins. The rates of resistance induced by clindamycin in erythromycin resistant (intermediate) and clindamycin sensitive strains are 25%, to carry out D test test can provide clinical reference data.