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目的:建立反相高效液相色谱法测定盐酸氟西汀胶囊的含量。方法:采用kromasil C18色谱柱(5μm,4.5mm×15mm)为分离柱;流动相为甲醇:0.05mol/L磷酸二氢钾缓冲液(55:45);流速:1mL／min;检测波长:230nm;柱温:室温。结果:盐酸氟西汀在52.5mg/L-315mg/L浓度范围内线性良好(r=0.9994);方法平均回收率为99.66％(RSD=0.98％,n=5);5次测定的精密度0.16％-0.35％。结论:本方法操作简便、快速、准确、灵敏,适用于盐酸氟西汀胶囊的含量测定。
Objective: To establish a RP-HPLC method for the determination of fluoxetine hydrochloride capsules. METHODS: Kromasil C18 column (5μm, 4.5mm × 15mm) was used as the separation column. The mobile phase consisted of methanol: 0.05mol / L potassium dihydrogen phosphate buffer (55:45), flow rate of 1mL / min, detection wavelength of 230nm Column temperature: room temperature. Results: Fluoxetine hydrochloride had a good linearity (r = 0.9994) in the range of 52.5mg / L to 315mg / L. The average recoveries were 99.66% (RSD = 0.98%, n = 5) 0.16% -0.35%. Conclusion: The method is simple, rapid, accurate and sensitive and is suitable for the determination of fluoxetine hydrochloride capsules.