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目的 通过流式细胞技术定量检测受低强度微波辐射后兔晶体上皮细胞的早期损伤改变来验证微波安全接触标准的可靠性并探讨其发生机理。方法 用强度分别为 5mW /cm2 和 10mW/cm2 的微波连续照射兔眼 3h ,流式细胞AnnexinⅤ PI(碘化丙啶 )双染色法定量检测兔晶体上皮细胞受辐射损伤的最早期变化 ,以自身防护眼作对照比较。结果 5mW /cm2 实验组兔晶体上皮细胞多数发生了早期凋亡。 10mW /cm2 实验组兔晶体上皮细胞受辐射后多数经早期凋亡发展到了细胞继发性坏死阶段。两组比较 ,10mW/cm2 的微波对细胞的损伤明显大于 5mW/cm2 的微波。结论 5mW /cm2 及 10mW /cm2 的低强度微波均导致兔晶体上皮细胞发生了不可逆的损伤 ,这种损伤是细胞水平上微波“非热”效应的结果。
Objective To quantitatively detect the early damage of rabbit lens epithelial cells after exposure to low-intensity microwave radiation by flow cytometry to verify the reliability of microwave safe contact standard and to explore its mechanism. Methods The rabbits were exposed to microwaves of 5 mW / cm2 and 10 mW / cm2 respectively for 3 h. The earliest changes of the rabbit lens epithelial cells were detected by flow cytometry with Annexin Ⅴ PI (propidium iodide) double staining. Eye for comparison. Results Most of the epithelial cells in the 5 mW / cm2 experimental group had early apoptosis. In the experimental group of 10 mW / cm2, most of the epithelial cells of the rabbit developed the stage of cell secondary necrosis by the early apoptosis after irradiation. The two groups, 10mW / cm2 microwave damage to cells was significantly greater than 5mW / cm2 microwave. Conclusion Low intensity microwaves of 5mW / cm2 and 10mW / cm2 all cause irreversible damage to rabbit lens epithelial cells. This damage is the result of microwave “non-thermal” effect at the cellular level.