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目的:研究黄芩苷与橙皮苷对顺铂所致小鼠肝损伤的保护作用,并比较二者的作用。方法:将ICR小鼠分为4组,即正常组、顺铂模型组、黄芩苷组和橙皮苷组,给药组分别给予黄芩苷(300mg/kg)和橙皮苷(300mg/kg),连续灌胃7天,正常组和模型组给予同体积溶剂,在第3天给药后1h时腹腔注射顺铂(5mg/kg)连续5天,末次注射15h后断头处死小鼠,取肝脏和血液进行检测,通过肝脏的病理组织学检测、谷丙转氨酶(ALT)、谷草转氨酶(AST)测定可反映顺铂引起肝脏损伤,利用生化法测定肝组织丙二醛(MDA)、超氧化物岐化酶(SOD)和谷胱甘肽(GSH)可反映其氧化损伤情况。结果:黄芩苷和橙皮苷可显著抑制顺铂引起小鼠血清ALT和AST水平升高,减少MDA的形成,恢复抗氧化酶SOD的活性,并抑制GSH的耗竭,但黄芩苷的作用比橙皮苷更显著。此外,黄芩苷和橙皮苷明显减轻肝脏水样空泡样变性、炎症浸润和坏死。结论:黄芩苷与橙皮苷均对顺铂所致小鼠肝损伤均具有明显保护作用,尤其是黄芩苷,其保护作用主要与抗氧化作用密切相关。
Objective: To study the protective effects of baicalin and hesperidin on liver injury induced by cisplatin in mice and to compare their effects. Methods: ICR mice were divided into four groups: normal group, cisplatin model group, baicalin group and hesperidin group. Baicalin (300mg / kg) and hesperidin (300mg / kg) The rats in the normal group and model group were given the same volume of solvent. The rats were sacrificed 5 days after the first injection of cisplatin (5mg / kg) Liver and blood were detected by the liver histopathological examination, alanine aminotransferase (ALT), aspartate aminotransferase (AST) determination of liver damage caused by cisplatin, the use of biochemical methods of liver malondialdehyde (MDA), superoxide Dioxide (SOD) and glutathione (GSH) can reflect the oxidative damage. Results: Baicalin and hesperidin significantly inhibited the increase of serum ALT and AST, reduced the formation of MDA, restored the activity of antioxidant enzyme SOD and inhibited the depletion of GSH in mice, but the effect of baicalin was stronger than that of orange Pigeon more significant. In addition, baicalin and hesperidin significantly reduce liver watery vacuolar degeneration, inflammatory infiltration and necrosis. CONCLUSION: Both baicalin and hesperidin have significant protective effects on cisplatin-induced liver injury in mice, especially baicalin. The protective effect of baicalin and hesperidin is mainly related to the antioxidation.