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【目的】通过对分离自云南德宏州的含羞草β-根瘤菌进行遗传与表型多样性研究,揭示我国含羞草β-根瘤菌的物种多样性。【方法】应用16S rDNA PCR-RFLP、全细胞蛋白SDS-PAGE电泳及16S rDNA全序列分析对分离得到的60株含羞草根瘤菌进行多样性研究。【结果】16S rDNA PCR-RFLP及全细胞蛋白SDS-PAGE图谱分析将供试菌株分为2个遗传型群和2个表型群,分别与贪铜菌属(Cupriavidus)和伯克霍尔德菌属(Burkholderia)参比菌株聚群。经16S rDNA全序列分析,供试菌株被归到台湾贪铜菌(Cupriavidus taiwanensis)、含羞草伯克霍尔德菌(Burkholderia mimosarum)及结瘤伯克霍尔德菌(Burkholderia phymatum)等3个种群。【结论】云南德宏州的含羞草β-根瘤菌主要为贪铜菌及伯克霍尔德菌类群,其中贪铜菌占绝对优势,且存在遗传和表型的丰富多样性,该研究揭示了含羞草β-根瘤菌的物种多样性并丰富了我国β-根瘤菌菌种资源。
【Objective】 The genetic diversity and phenotypic diversity of mimosa β-Rhizobium isolated from Dehong Prefecture in Yunnan Province were studied to reveal the species diversity of mimosa β-Rhizobia in China. 【Method】 The diversity of 60 strains of Mimosaceae Rhizobia isolated by 16S rDNA PCR-RFLP, whole cell protein SDS-PAGE and 16S rDNA sequence analysis were studied. 【Result】 The 16S rDNA PCR-RFLP and whole-cell protein SDS-PAGE analysis of the strains were divided into two genetic groups and two phenotypic groups, respectively, Cupriavidus and Burkholde Burkholderia reference strains are clustered. After 16S rDNA sequence analysis, the tested strains were classified into three species, Cupriavidus taiwanensis, Burkholderia mimosarum and Burkholderia phymatum Population. 【Conclusion】 The β-Rhizobium mimosas in Dehong prefecture of Yunnan Province are mainly Bacillus brassoris and Burkholderia species, of which copper-dominated bacteria dominate, and there is rich genetic and phenotypic diversity. Revealed the species diversity of mimosa β-Rhizobia and enriched the β Rhizobium species in China.