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目的:建立RP-HPLC同步测定延胡索中11种生物碱成分的方法,为该药材的质量控制提供参考。方法:Kromasil Eternity C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.1 mol·L-1乙酸铵溶液(每1 L溶液中加冰乙酸0.5m L)(B)梯度洗脱(0~50 min,20%~55%A;50~55 min,55%~65%A;55~60 min,65%A),流速1.0 m L·min-1,柱温25℃,检测波长280 nm,进样量10μL。结果:11种待测成分的线性关系良好,相关系数均>0.999;精密度试验RSD 0.1%~2.0%,稳定性试验RSD 0.2%~2.1%,重复性试验RSD 1.7%~4.1%,平均加样回收率98.2%~100.8%(RSD 1.6%~3.5%),均符合中药质量分析要求。结论:建立的RP-HPLC灵敏、简便、可靠,可用于延胡索药材中多种生物碱成分的同步测定。
OBJECTIVE: To establish a method for simultaneous determination of 11 alkaloids in Corydalis yanhusuo by RP-HPLC and to provide references for the quality control of this herb. METHODS: A gradient of Kromasil Eternity C18 column (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile (A) -0.1 mol·L -1 ammonium acetate solution (0.5 mL of glacial acetic acid per 1 L of solution) (0-50 min, 20% -55% A; 50-55 min, 55% -65% A; 55-60 min, 65% A) at a flow rate of 1.0 m L · min- , Detection wavelength 280 nm, injection volume 10μL. Results: The linear relationship between the 11 tested components was good, the correlation coefficients were all> 0.999; RSD of precision test was 0.1% ~ 2.0%, RSD of stability test was 0.2% ~ 2.1%, RSD of repeatability test was 1.7% ~ 4.1% The recoveries ranged from 98.2% to 100.8% (RSD 1.6% ~ 3.5%), which met the requirements of Chinese medicine quality analysis. Conclusion: The established RP-HPLC is sensitive, simple and reliable and can be used for simultaneous determination of a variety of alkaloids in the Corydalis yanhusuo.