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本文应用高效液相色谱法测定人血浆中头孢唑啉的浓度，以头孢哌酮为内标，醋酸—醋酸铵的甲醇溶液（ｐＨ４．３５，离子强度０．０２ｍｏｌ／Ｌ）为流动相，紫外２７０ｎｍ检测。头孢唑啉平均回收率为：９４．７４±６．２６％，其日间误差和日内误差（ＲＳＤ％）均小于５％，在８～２００μｇ／ｍｌ浓度范围内线性良好，相关系数ｒ＝０．９９９９。应用本法测定头孢唑啉静脉滴注和肌注间血药浓度的差别，发现肌注也能够达到同样冲击峰浓度，而且能够节约药品用量并降低工作量。对调整方案，减少毒副作用的发生具有临床意义。
In this paper, HPLC was used to determine the concentration of cefazolin in human plasma. Cefoperazone was used as internal standard. Acetic acid - ammonium acetate in methanol (pH4.35, ionic strength 0.02mol / L) was used as the mobile phase. 270nm detection. The average recoveries of cefazolin were 94.74 ± 6.26%. The intra-day errors and intra-day errors (RSD%) were all less than 5%. The linearity was good at a concentration range of 8-200 μg / ml with a correlation coefficient of r = 0 .9999. This method was used to determine the difference between cefazolin intravenous drip and intramuscular injection of blood concentration and found that intramuscular injection can achieve the same impact peak concentration, and can save the amount of drugs and reduce the workload. On the adjustment program to reduce the incidence of toxic and side effects of clinical significance.