论文部分内容阅读
目的:探讨长链非编码RNA XLOC_005009基因对食管鳞状细胞癌(ESCC)的体外增殖、迁移、侵袭和细胞周期与细胞凋亡等生物学特性的影响。方法:收集2015-2016年河北医科大学第四医院肿瘤研究所57例ESCC患者手术切除的癌组织及癌旁组织标本。应用实时荧光定量PCR检测XLOC_005009基因在人ESCC细胞系Eca109、Kyse170与ESCC组织及其癌旁组织的表达,构建pc DNA3.1-XLOC_005009过表达质粒并转染Eca109、Kyse170细胞,应用MTS法、克隆形成实验、划痕实验、Transwell小室、流式细胞术分别检测转染前后细胞增殖、克隆形成、迁移、侵袭、细胞周期与凋亡的变化。结果:XLOC_005009 m RNA在食管癌组织中的表达明显低于癌旁组织(0.06±0.06 vs 0.21±0.19,P<0.05),且食管癌细胞XLOC_005009 m RNA表达亦均低于对照组(P<0.05)。转染过表达质粒的Eca109和Kyse170细胞XLOC_005009基因表达显著高于对照组(Eca109细胞:039±0.17 vs0.02±0.00;Kyse170细胞:0.35±0.08 vs 0.01±0.01,均P<0.05)。与对照组相比,XLOC_005009基因过表达Eca109和Kyse170细胞增殖能力细胞显著减弱(P<0.05);克隆形成率显著减少(P<0.05);穿膜细胞数显著减少[Eca109细胞:(146.40±34.47)vs(193.00±26.33);Kyse170细胞:(157.80±32.51)vs(269.00±29.89),均P<0.05];Eca109细胞迁移率无显著变化,Kyse170细胞迁移率明显减小(P<0.05);S期细胞比例增加,细胞凋亡影响不明显。结论:XLOC_005009低表达与食管癌的发生发展密切相关,XLOC_005009过表达可抑制食管癌细胞的体外增殖、侵袭与迁移能力。
Objective: To investigate the effects of long-chain non-coding RNA XLOC_005009 on biological characteristics of esophageal squamous cell carcinoma (ESCC) in vitro such as proliferation, migration, invasion and cell cycle and apoptosis. Methods: Surgical resection of cancer tissues and adjacent tissues in 57 ESCC patients from 2015 to 2016 in the Fourth Hospital of Hebei Medical University were collected. The expression of XLOC_005009 gene in human ESCC cell lines Eca109, Kyse170 and ESCC tissues and their paracancerous tissues was detected by real-time fluorescence quantitative PCR. The pcDNA3.1-XLOC_005009 overexpression plasmid was constructed and transfected into Eca109 and Kyse170 cells. The MTS method and cloning The formation of experiments, scratch experiments, Transwell chamber, flow cytometry were detected before and after transfection cell proliferation, colony formation, migration, invasion, changes in cell cycle and apoptosis. Results: The expression of XLOC_005009 m RNA in esophageal cancer tissue was significantly lower than that in adjacent tissues (0.06 ± 0.06 vs 0.21 ± 0.19, P <0.05), and the expression of XLOC_005009 m RNA in esophageal cancer cells was also lower than that of the control group (P <0.05 ). The gene expression of XLOC_005009 in Eca109 and Kyse170 transfected with plasmid was significantly higher than that in control (Eca109: 039 ± 0.17 vs 0.02 ± 0.00; Kyse170: 0.35 ± 0.08 vs 0.01 ± 0.01, all P <0.05). Compared with the control group, XLOC_005009 gene overexpression Eca109 and Kyse170 cell proliferation significantly decreased (P <0.05); the colony formation rate was significantly reduced (P <0.05); the number of transmembrane cells was significantly decreased [Eca109 cells: (146.40 ± 34.47 (193.00 ± 26.33), Kyse170 cells (157.80 ± 32.51) vs (269.00 ± 29.89), all P <0.05]. There was no significant change in the migration of Eca109 cells and the migration of Kyse170 cells was significantly decreased (P <0.05) S phase cells increased, the impact of apoptosis was not obvious. Conclusion: The low expression of XLOC_005009 is closely related to the occurrence and development of esophageal cancer. Overexpression of XLOC_005009 can inhibit the proliferation, invasion and migration of esophageal cancer cells in vitro.