论文部分内容阅读
以耐海水菠菜品种‘荷兰3号’为材料,采用水培方法,研究了二硫苏糖醇(DTT)对海水胁迫及甲基紫精(MV)诱导下菠菜活性氧代谢及叶绿素荧光特性的影响。结果表明,海水胁迫与MV处理一样,诱导菠菜叶片产生氧化胁迫,使超氧阴离子( )产生速率、过氧化氢(H2O2)含量和丙二醛(MDA)含量显著上升,叶绿素a(Chl.a)、叶绿素b(Chl.b)、总叶绿素[Chl.(a+b)]和类胡萝卜素(Car.)含量显著下降,最大光量子产量(Fv/Fm)、实际光量子产量(Yield)、电子传递速率(ETR)和光化学猝灭系数(qP)显著降低,而非光化学猝灭系数(NPQ/4)显著上升;海水胁迫与MV处理下,由叶柄导入叶黄素循环活性抑制剂DTT,菠菜叶片活性氧(ROS)大量积累,导致光合色素降解加剧,Fv/Fm、Yield、ETR、NPQ、qP进一步下降。上述结果表明,海水胁迫抑制了菠菜叶片叶黄素循环活性,降低了叶片非辐射能量耗散能力,加重了叶片ROS积累,从而导致光合色素含量降低,PSⅡ活性下降,电子传递速率降低,用于光化学反应的能量部分减少,光合作用受到严重影响,说明海水胁迫下叶黄素循环在保持菠菜叶片光合色素稳定和光合作用正常运转中发挥重要作用。
Using spinach cultivar “Holland No. 3” as material, the effects of dithiothreitol (DTT) on seawater stress and reactive oxygen metabolism and chlorophyll fluorescence characteristics of spinach induced by methyl viologen (MV) were studied. influences. The results showed that seawater stress induced the oxidative stress on spinach leaves as well as MV treatment, and the superoxide anion production rate, hydrogen peroxide (H2O2) content and malondialdehyde (MDA) content were significantly increased, and chlorophyll a (Chl.a ), Chlorophyll b (Chl.b), total chlorophyll [Chl. (A + b)] and carotenoid content decreased significantly, the maximum photochemical quantum yield (Fv / Fm), actual photochemical yield, (ETR) and photochemical quenching coefficient (qP) were significantly decreased, but non-photochemical quenching coefficient (NPQ / 4) increased significantly. Under seawater stress and MV treatment, the leaf lutein cycle active inhibitor DTT, spinach The accumulation of reactive oxygen species (ROS) in leaves caused more degradation of photosynthetic pigments and further decreased Fv / Fm, Yield, ETR, NPQ and qP. The results showed that seawater stress inhibited the xanthophyll cycle activity of spinach leaves, reduced the non-radiative energy dissipation ability of leaves and increased the accumulation of ROS, resulting in the decrease of photosynthetic pigment content, the decrease of PSⅡ activity and the decrease of electron transfer rate, Photochemical reaction part of the reduction in energy, photosynthesis was severely affected, indicating that the seawater stress lutein cycle in maintaining spinach leaves photosynthetic pigment stability and photosynthesis play an important role in the normal operation.