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运用硅胶、Sephadex LH-20柱色谱和HPLC制备色谱等方法进行分离和纯化,从茜草科红芽大戟属植物红大戟根的乙醇提取物中首次分离得到21个非蒽醌类成分;通过NMR和MS等波谱数据鉴定了化合物的结构,包括10个三萜:乌苏酸(1),齐墩果酸(2),3β,19α-二羟基-2-氧-乌苏-12-烯-28-酸(3),坡模酸(4),马斯里酸(5),3β,19α,24-三羟基-乌苏-12-烯-28-酸(6),委陵菜酸(7),救必应酸-3,23-缩丙酮(8),2α,3β,19α,23-四羟基-齐墩果-12-烯-28-酸(9),2α,3β,19α,23-四羟基-乌苏-12-烯-28-酸(10);4个豆甾酮:(24R)-24-豆甾-4,22-二烯-3-酮(11),(24R)-24-豆甾-4-烯-3-酮(12),(24R)-24-豆甾-3β-羟基-5,22-二烯-7-酮(13),(24R)-24-豆甾-3β-羟基-5-烯-7-酮(14);2个木脂素:桉脂素(15),刺五加酮(16);1个香豆素:8-甲氧基异欧前胡素(17);4个简单芳香类化合物:5-羟甲基呋喃醛(18),3-羟基-4-甲氧基苯甲酸(19),苯甲酸(20),2-羟基-5-甲氧基-苯丙烯醛(21)。在肿瘤细胞毒(MTT法,HCT-8,Bel7402,BGC-823,A549和A2780),神经细胞保护(去血清和谷氨酸损伤模型),抗氧化(Fe2+-Cys诱导大鼠肝微粒体丙二醛生成模型),抗炎(小鼠腹腔巨噬细胞分泌NO模型),抗HIV(VS-VG/HIV-luc模型)和抗糖尿病(PTP1B酶抑制模型)药理模型上筛选结果显示,在1.0×10-5mol.L-1浓度下,这些化合物均未表现出活性。
Separation and purification were carried out by silica gel, Sephadex LH-20 column chromatography and HPLC preparative chromatography. 21 non-anthraquinone components were isolated from the ethanol extract of Rubus Euphorbia root, The structures of the compounds were identified by NMR and MS spectra, including 10 triterpenes: ursolic acid (1), oleanolic acid (2), 3β, 19α-dihydroxy-2-oxo-uesu-12-ene (6), cephalannic acid (4), masriolic acid (5), 3β, 19α, 24-trihydroxy-uesu-12-en-28- (8), 2α, 3β, 19α, 23-tetrahydroxy-olean-12-en-28-oic acid (9), 2α, 3β, 19α 4-stigmasterone: (24R) -24-stigmaster-4,22-dien-3-one (11), and 24R) -24-stigmaster-3β-hydroxy-5,22-dien-7-one (13), (24R) 3-hydroxy-5-en-7-one (14); 2 lignans: eucalyptol (15), capu penta ketone (16); 1 coumarin: 8- (17); 4 simple aromatic compounds: 5-hydroxymethylfuranal (18), 3-hydroxy-4- methoxybenzoic acid (19), benzoic acid 2-Hydroxy-5-methoxy-propenal (21). Neuronal cell protection (de-serum and glutamate-induced injury models), anti-oxidant (Fe2 + -Cys-induced rat liver microsomes C) were induced by tumor cytotoxicity (MTT assay, HCT-8, Bel7402, BGC-823, A549 and A2780) Dialdehyde formation model), anti-inflammatory (NO secretion model in mouse peritoneal macrophages), anti-HIV (VS-VG / HIV-luc model) and anti-diabetic (PTP1B enzyme inhibition model) pharmacological models showed that at 1.0 None of these compounds showed activity at concentrations of 10-5 mol.L-1.