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微流控芯片上的细胞培养技术是构建一个整体化的片上细胞分析实验室的基础。针对芯片上细胞培养存活率低、容易污染等问题,该文提出了一套polydimethylsiloxane(PDMS)-玻璃微流控芯片的制备、处理方法及片上细胞培养的操作流程,并讨论了影响微管道细胞培养的因素,包括准备细胞悬液、避免气泡、芯片表面处理和培养液中的血清浓度等。应用这套方法,在微流控芯片上成功培养了多种哺乳动物细胞。
Cell culture technology on microfluidic chips is the foundation for building an integrated on-chip cell analysis laboratory. In order to solve the problems of low viability and easy contamination of on-chip cell culture, a set of polydimethylsiloxane (PDMS) -glass microfluidic chip was prepared, the processing method and on-chip cell culture protocol were discussed. Incubation factors, including preparation of cell suspension, to avoid air bubbles, chip surface treatment and culture medium serum concentration. Using this method, a variety of mammalian cells have been successfully cultured on a microfluidic chip.