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目的 研究N 甲基亚硝基脲 (MNU)制备方法及其诱发大白鼠膀胱肿瘤的作用。方法由甲胺盐酸盐合成诱癌剂MNU ,进行大白鼠膀胱灌注 2mg/次 ,每 2周 1次 ,共 4次 ,以光镜、扫描电镜、透射电镜等为观察手段 ,对其诱发的膀胱肿瘤进行观察。结果 8周诱发膀胱肿瘤率为 10 0 % ,其组织学检查及病理学特征与人膀胱肿瘤十分相似。结论 自制的MNU诱发的大白鼠膀胱肿瘤为比较理想的动物模型。
Objective To study the preparation of N-methylnitrosourea (MNU) and its effect on bladder tumor in rats. Methods MNU was induced by methylamine hydrochloride and intravesical instillation of rats was performed at a dose of 2mg / time and once every 2 weeks for 4 times. Light microscopy, scanning electron microscopy and transmission electron microscopy were used as the observation tools. Bladder tumors were observed. Results The bladder tumor rate was 10% after 8 weeks. The histological and pathological features were similar to those of human bladder cancer. Conclusion The homemade MNU-induced bladder tumor in rats is an ideal animal model.