本研究采用体外培养兔关节软骨细胞的方法,建立黄嘌呤-黄嘌呤氧化酶系产生的内源性自由基对软骨细胞影响的实验模型。用倒置显微镜动态观察,扫描及透射电镜超微结构观察等研究方法,初步探讨了内源性自由基对兔关节软骨细胞的影响。结果表明:黄嘌呤-黄嘌呤氧化酶系产生的内源性自由基对软骨细胞具有损伤作用。提示软骨老化、变性、一些变性性关节疾病,如大骨节病、关节炎的发生可能与自由基有关。 In this study, rabbit articular chondrocytes cultured in vitro methods to establish xanthine - xanthine oxidase-derived endogenous free radicals on chondrocytes experimental model. The effects of endogenous free radicals on rabbit articular chondrocytes were preliminarily investigated by dynamic observation of inverted microscope, scanning and transmission electron microscopy. The results showed that xanthine - xanthine oxidase produced endogenous free radicals on chondrocytes injury. Tips cartilage aging, degeneration, some degenerative joint diseases, such as Kashin-Beck disease, the incidence of arthritis may be related to free radicals.