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目的 :测定水合氯醛对大肠杆菌和金黄色葡萄球菌的MIC、MBC ,并考察培养基种类对测定结果的影响 ,探讨不同MBC测定方法的优缺点。方法 :液体培养基两倍稀释法 ,以胆盐乳糖和营养肉汤测定水合氯醛对大肠杆菌和金黄色葡萄球菌的MIC ,并以不同的方法测定MBC。结果 :对大肠杆菌两种培养基中的MIC均为 3.12 5× 10 -3 g·ml-1,3种不同的方法所测定的MBC均为 6 .2 5× 10 -3 g·ml-1,对金黄色葡萄球菌 ,在营养肉汤中MIC为 6 .2 5× 10 -3 g·ml-1,3种不同的方法所测定的MBC均为 6 .2 5× 10 -3 g·ml-1,而在胆盐乳糖中其MIC、MBC均不能测出。结论 :水合氯醛对大肠杆菌、金黄色葡萄球菌有较强的抗菌作用 ,对G-的大肠杆菌 ,两种培养基对MIC、MBC结果无影响 ,但对G+ 的金黄色葡萄球菌 ,胆盐乳糖培养基可抑制其生长而影响其MIC、MBC的测出 ,不同的MBC测定方法虽各有利弊 ,但本实验的测定结果一致。
OBJECTIVE: To determine the MIC and MBC of chloral hydrate on Escherichia coli and Staphylococcus aureus and investigate the effect of culture medium on the determination results. The advantages and disadvantages of different MBC determination methods were discussed. Methods: Two-fold dilution of liquid medium was used to determine the MIC of chloral hydrate to E. coli and Staphylococcus aureus with bile-salt lactose and nutrient broth. MBC was determined by different methods. Results: The MICs of E. coli in both culture media were 3.12 5 × 10 -3 g · ml-1, and the MBCs determined by different methods were all 6.52 × 10 -3 g · ml -1 , For the Staphylococcus aureus, the MBC in the nutrient broth was 6.52 × 10 -3 g · ml -1, and the MBCs determined by different methods were all 6.52 × 10 -3 g · ml -1, while in the bile salt lactose its MIC, MBC can not be measured. Conclusion: Chloral hydrate has a strong antibacterial effect on Escherichia coli and Staphylococcus aureus. For G-Escherichia coli, the two mediums have no effect on the MIC and MBC results. However, for Staphylococcus aureus and bile salts Lactose medium can inhibit its growth and affect its MIC, MBC measured, although different methods of determination of MBC have their own advantages and disadvantages, but the experimental determination of the same.