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粤晶丝苗2号是广东省优质稻主栽品种和省区试对照品种,具有高度的稻瘟病抗性.用中国不同地区297个稻瘟病菌株进行抗谱分析,粤晶丝苗2号对广东、四川、辽宁和黑龙江稻瘟病菌群体的抗性频率均达到100%,其抗谱较主要抗源品种三黄占和28占更广.利用不同的菌株,对粤晶丝苗2号/露明的F2群体和F4株系进行抗性分离分析,结果表明,该品种的稻瘟病抗性由显、隐性的多基因控制.通过基于F4单基因分离株系的BSA分析,分别在染色体2,6,8和12上筛选到了与其抗病基因连锁的候选标记.通过基于基因组位置已知的分子标记的连锁分析,将其中的隐性基因定位于染色体8约1.9cM/152kb的区域内.由于该区域内尚未有主效抗病基因的存在,因此,该基因是新的隐性抗病基因,被命名为pi55(t).通过对该区域内候选基因的注释,发现其中编码LRR蛋白的Os08g40090和编码重金属结合域蛋白的Os08g40130可能是其最佳的候选基因.
Yuejing Si Miao 2 is a high-quality rice cultivars and provincial control varieties in Guangdong Province, with a high degree of resistance to blast.The anti-spectrum analysis of 297 blast strains in different regions of China, Yue Jing Si Miao No. 2 The resistance frequency of Magnaporthe grisea in Guangdong, Sichuan, Liaoning and Heilongjiang reached 100%, and their resistance spectrum was broader than that of Sanhuangzhanzhan 28, which is the main source of resistance.With different strains, The results showed that the blast resistance of this strain was controlled by the dominant and recessive polygenes.According to the analysis of BSA based on F4 single gene isolates, 2, 6, 8 and 12. Candidate markers linked to their disease-resistance genes were screened on the basis of linkage analysis of known molecular markers based on their genomic positions, and the recessive genes were located in the region of about 1.9cM / 152kb on chromosome 8 .This gene is a new recessive disease-resistance gene, named pi55 (t), because there is no major resistance gene in this region.After annotating the candidate genes in this region, it was found that the gene encoding LRR Os08g40090 encoding proteins and Os08g4 encoding heavy metal binding domain proteins 0130 may be its best candidate gene.