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为了建立一个体内标记血吸虫尾蚴的标准技术,作者用~(75)Se-甲硫氨酸分别标记感染曼氏血吸虫的扁卷螺(B.glabrata)和感染间插血吸虫的膀胱螺(B.forskalii),这个方法可以保证从螺蛳中连续逸出标记尾蚴,而且不影响标记尾蚴的产量和对动物宿主的感染力。作者同时探讨了影响标记的一系列因素。标记方法归纳如下: 以扁卷螺(壳的直径2~3毫米)为例,每螺感染曼氏血吸虫毛锄8条,约6周后,选择尾蚴逸出量特别高的螺蛳,分别放在含有
To establish a standard technique for in vivo labeling of Schistosoma japonicum cercariae, the authors labeled B.glabrata infected with Schistosoma mansoni and B.forskalii infected with S.interpunctatus using ~ (75) Se-methionine, respectively ). This method ensures that the labeled cercariae continue to escape from the snails without affecting the yield of labeled cercariae and the infectivity of the animal host. The author also explored a series of factors that influence the markup. Marked methods are summarized as follows: Flat snail (shell diameter of 2 to 3 mm), for example, each infected with Schistosoma mansoni hairy 8, about 6 weeks later, select the cercariae escaped particularly high snail, were placed in contain