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对２３例国内献血员、血透析及肝炎病人血清用反转录巢式ＰＣＲ技术扩增了ＨＣＶＲＮＡ囊膜蛋白２基因的ｃＤＮＡ片段，并进行了序列测定。结果表明２３例病人ＨＣＶＥ２／ＮＳ１Ｎ末端的核苷酸及氨基酸序列呈现多样性，高变区１（ＨＶＲ１）位于核苷酸第１４５９～１５５９位，氨基酸第３８４～４１０位；我国ＨＣＶ株ＨＶＲ１２７个氨基酸中有１５个位置氨基酸相对稳定，氨基酸组成与分布均与Ｓｅｋｉｙａ报道的１６６个ＨＣＶ株的不同。结果提示，研究我国ＨＣＶ株ＨＶＲ１的序列特征有助于ＨＣＶ的流行病学研究，对研制适用于我国的抗体诊断试剂盒及进行疫苗研究均有重要的意义。
23 cDNA fragments of HCVRNA envelope protein 2 gene were amplified by reverse transcription nested PCR from 23 blood donors in China, hemodialysis patients and hepatitis patients, and sequenced. The results showed that the nucleotide and amino acid sequences of the HCVE2 / NS1 N terminal showed diversity in 23 patients. HVR1 was located at nucleotide 1459-1559 and amino acid 384-410. HVR127 amino acids 15 amino acids were relatively stable, the amino acid composition and distribution are different from the 166 HCV strains reported by Sekiya. The results suggest that the study of the HVR1 sequence of HCV strains in China is helpful to the epidemiological study of HCV and is of great significance to the development of antibody diagnostic kits and vaccine research in our country.