论文部分内容阅读
目的:研究生长激素1(growth hormone1,GH1)基因T1663A多态性与结直肠癌易感性的关系。方法:在江苏省进行了一个病例-对照研究(结直肠癌患者315例,人群对照439名),调查研究对象的生活习惯,抽取静脉血,提取白细胞DNA,采用PCR-RFLP检测研究对象的GH1T1663A基因型。结果:1)GH1T/T、T/A和A/A基因型分布频度在结直肠癌组分别为42.2%、46.7%和11.1%,对照组分别为38·1%、45·9%和16·0%,两组差异无统计学意义,χ2MH=3·907,P=0·142。但在调整性别、年龄、吸烟和饮酒习惯后,A/A基因型者与T/T基因型者相比,发生结直肠癌的危险性显著降低(OR=0·88,95%CI:0·78~0·99,P=0·0287)。2)多因素分析结果显示,饮酒者患结直肠癌的危险性显著增高(OR=1·96,95%CI:1·34~2·86,P=0·0005),A/A基因型与降低结直肠癌的危险性有关,而吸烟与增加或降低结直肠癌的危险性无显著相关。3)GH1基因多态与吸烟、饮酒相互作用的分层分析发现,在不吸烟者中,GH1A/A基因型者与T等位基因型者相比,发生结直肠癌的危险性显著降低(性别和年龄调整OR=0·50,95%CI:0·27~0·93);在不饮酒者中,GH1A/A基因型者发生结直肠癌的调整OR为0·56(95%CI:0·32~0·99)。结论:T1663A GH1基因的A/A基因型可降低结直肠癌易感性,特别是在不吸烟和不饮酒者中。
Objective: To investigate the relationship between T1663A polymorphism of growth hormone 1 (GH1) gene and susceptibility to colorectal cancer. METHODS: A case-control study (315 colorectal cancer patients and 439 population controls) was conducted in Jiangsu Province. The living habits of the study subjects were investigated. Venous blood was collected for leucocyte DNA extraction. PCR-RFLP was used to detect the GH1T1663A genotype. Results: 1) The frequencies of GH1T / T, T / A and A / A genotypes were 42.2%, 46.7% and 11.1% in the colorectal cancer group and 38.1% and 45.9% in the control group 16.0%, no significant difference between the two groups, χ2MH = 3.907, P = 0.142. However, the risk of developing colorectal cancer was significantly lower in those with A / A genotypes than those with genotype T / T after adjustment for gender, age, smoking, and alcohol consumption (OR = 0.88, 95% CI: 0 · 78 ~ 0 · 99, P = 0 · 0287). 2) The results of multivariate analysis showed that the risk of colorectal cancer was significantly higher in drinkers (OR = 1.96, 95% CI: 1.34-2.286, P = 0.0005). The A / A genotype There was no significant correlation between smoking and the risk of increasing or decreasing colorectal cancer. 3) Hierarchical analysis of the interaction between GH1 gene polymorphisms and smoking, drinking and drinking found that in non-smokers, the risk of colorectal cancer was significantly lower in GH1A / A genotype than in T allele ( Gender and age adjusted OR = 0.50, 95% CI: 0.27-0.93). In non-drinkers, the adjusted OR for colorectal cancer with GH1A / A genotype was 0.56 (95% CI : 0 · 32 ~ 0 · 99). CONCLUSION: The A / A genotype of the T1663A GH1 gene reduces susceptibility to colorectal cancer, especially in non-smokers and non-drinkers.