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背景:脑内一些区域内胶质细胞中铁的过多沉积和由铁介导的氧化应激反应可能与帕金森病等一些神经退行性疾病的发病有关,但目前对老年F344大鼠中脑黑质内铁沉积的亚细胞结构研究客观评价标准并不多见。目的:观察老年F344大鼠黑质部位铁的沉积,为进一步了解PD的发病机制提供理论依据。设计:随机对照实验研究。单位:首都医科大学北京神经科学研究所和DepartmentofAnatomy&Neurobiology,UniversityofKentucky,CollegeofMedicine,LexintonU.S.A。材料:选择24个月(6只),3个月(4只)Fischer(F344)雄性大鼠。干预:将灌注固定后的脑组织取出中脑部分置于振动切片机上,将其切成40μm脑薄片。分别采用免疫组织化学方法:激光共聚焦显微镜;透射电子显微镜及电子探针元素能量微分析等技术,对中脑黑质部位铁的沉积做一系统组织化学及形态学观察。主要观察指标:在光镜、激光共聚焦显微镜、透射电子显微镜下观察及电子探针元素能量微分析中脑黑质部位铁的沉积。结果:在光镜下,作为铁的主要储存形式-铁结合蛋白在黑质的网状部有明显增加并伴随着小胶质的增加,激光共聚焦显微镜下观察到它们很少存在于多巴胺神经元及星形质细胞内,而主要存在于小胶质中,利用电子探针检测结果显示的游离铁元素,主要位于星形胶质细胞的胞浆内,?
BACKGROUND: Excessive iron deposition and iron-mediated oxidative stress in glial cells in some regions of the brain may be related to the pathogenesis of some neurodegenerative diseases such as Parkinson’s disease. However, Subjective evaluation criteria for subcellular localization of iron deposition in the interior are rare. OBJECTIVE: To observe the deposition of iron in the substantia nigra of aged F344 rats and provide a theoretical basis for further understanding of the pathogenesis of PD. Design: Randomized controlled trial. Organization: Beijing Institute of Neuroscience, Capital Medical University and Department of Anatomy & Neurobiology, University of Kentucky, College of Medicine, Lexinton U.S.A. MATERIALS: Fischer (F344) male rats were selected 24 months (6 mice) and 3 months (4 mice). Intervention: The perfusion-fixed brain tissue was removed from the midbrain part on a vibrating microtome and cut into 40 μm brain slices. Immunohistochemistry and laser scanning confocal microscopy were used to observe the deposition of iron in the substantia nigra of the midbrain through the techniques of transmission electron microscopy (TEM) and electron probe microanalysis. MAIN OUTCOME MEASURES: The deposition of iron in the substantia nigra was observed under light microscope, confocal laser scanning microscope and transmission electron microscopy and energy microanalysis of electron probe element. RESULTS: Under the light microscope, as the main storage form of iron, iron-binding protein in the substantia nigra reticular part increased significantly with the increase of microglia, they were rarely seen in laser scanning confocal microscopy in dopamine nerve Yuan and astrocytes, but mainly in microglia, the use of electron probe detection results show that the free iron, mainly in the astrocyte cytoplasm,?