微纳复合结构对成纤维细胞黏附增殖的影响

来源 :临床口腔医学杂志 | 被引量 : 0次 | 上传用户:j443191910
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目的:探讨经不同方法处理后的纯钛表面对成纤维细胞黏附增殖的影响。方法:将36个试件分平均为3组:机械抛光组(A组);喷砂酸蚀组(B组);喷砂酸蚀碱热组(C组),每组均12个试件。通过扫描电子显微镜(SEM)观察分析3组试件表面微观结构和细胞在试件表面的铺展情况,激光共聚焦显微镜(CLSM)检测各试件表面的粗糙度;运用CCK-8试剂盒在450 nm波长下检测各试件对成纤维细胞(L929)黏附与增殖的吸光度值(OD值)。结果:A组表面光滑,试件表面成纤维细胞骨架大多呈梭形铺展,伸展较差;B组和C组表面粗糙,且C组表面可见微纳复合结构,试件表面成纤维细胞骨架呈三维空间向铺展,表面黏附成纤维细胞数量明显多于A组和B组。观察第1,3,5 d试件表面细胞增殖情况,可见粗糙表面较光滑表面更利于成纤维细胞的增殖。结论:喷砂酸蚀碱热方法处理后的纯钛表面形成微米-纳米复合孔洞,表面活性好,促进成纤维细胞早期黏附及表面铺展,且不抑制细胞的增殖。 Objective: To investigate the effect of different treatments on the adhesion and proliferation of fibroblasts on pure titanium surface. Methods: 36 specimens were divided into three groups on average: mechanical polishing group (group A), sandblasting acid etching group (group B), sandblasting acid etching alkali thermal group (group C) . The microstructure of the three groups of samples and the spreading of the cells on the surface of the specimens were observed by scanning electron microscope (SEM). The surface roughness of each specimen was detected by laser confocal microscopy (CLSM) The absorbance value (OD value) of each specimen on the adhesion and proliferation of fibroblasts (L929) was detected under the wavelength of nm. Results: The surface of group A was smooth. Most of the fibroblast cytoskeleton on the surface of the specimen showed spindle-shaped spreading and poor stretching. The surface of group B and C were rough, and the surface of group C was micro / nano composite. The surface of fibroblast was Three-dimensional space to spread, the surface of the number of adherent fibroblasts significantly more than A and B group. The proliferation of cells on the 1st, 3rd, 5th day specimens was observed, showing that the rougher surface is more conducive to the proliferation of fibroblasts than the smooth surface. CONCLUSION: The surface of pure titanium treated by sandblasting and acidizing alkali treatment can form micrometer - nanocomposite pores with good surface activity and promote the early adhesion and surface spreading of fibroblasts without inhibiting the proliferation of cells.
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