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目的比较灭能与未灭能新生牛血清培养Vero细胞和MRC-5细胞的效果,为细胞培养的相关研究和生产提供参考。方法分别采用照射剂量为8和16 k Gy的60Coγ-射线及56℃30 min加热灭能新生牛血清,用两种方式灭能的血清及未灭能的新生牛血清培养Vero细胞和MRC-5细胞,显微镜观察细胞的生长情况,计数并计算细胞倍增时间及存活时间。结果未灭能新生牛血清培养的两种细胞增殖更多更快速;热灭能的新生牛血清培养的两种细胞的增殖速度和数量明显低于γ-射线照射灭能及未灭能血清培养的细胞。结论未灭能的新生牛血清比灭能后的血清培养Vero细胞和MRC-5细胞的效果好,其促细胞生长增殖效果最佳。
OBJECTIVE: To compare the effect of neonatal bovine serum with and without extinct on Vero cells and MRC-5 cells, and provide reference for the related research and production of cell culture. METHODS: Vero cells and MRC-5 cells were cultured in 60Co γ-rays irradiated at 8 and 16 k Gy and heat-inactivated neonatal bovine serum at 56 ℃ for 30 min, respectively. Two methods of inactivating serum and non-inactivated neonatal bovine serum Cells, microscopic observation of cell growth, counting and calculation of cell doubling time and survival time. Results Both of the two kinds of cells cultured in non-quenched newborn calf serum proliferated more and more rapidly. The proliferation rate and number of the two kinds of cells cultured in heat-killed neonatal calf serum were significantly lower than those in γ-ray irradiation and non-quenched serum Cell. Conclusion The newborn calf serum which is not quenched has a better effect on culturing Vero cells and MRC-5 cells than the serum after being killed, and has the best effect on promoting cell proliferation.