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目的:探讨小鼠乳腺癌化疗、放疗后存活顿抑癌细胞的超微结构特征。方法:TAⅡ乳腺癌小鼠36只,随机分为对照组、化疗组(环磷酰胺、5-氟脲嘧啶、氨甲喋呤)和放疗组(X射线),用电镜及电脑图像分析系统对治疗后残留的存活癌细胞进行形态分析。结果:有效化疗和放疗后的癌组织绝大多数癌细胞已死亡,在坏死癌组织中仍残留着形态完好的癌细胞。结构上分析这类细胞仍然存活,将其命名为顿抑癌细胞,并分为两类:Ⅰ型顿抑癌细胞和Ⅱ型顿抑癌细胞。顿抑癌细胞的平均体积明显小于对照组(P<0.001),线粒体和粗面内质网的体密度也明显少于对照组(P<0.001或P<0.01),残留的顿抑癌细胞在对照组中也找到了同形的相应的副本对应细胞。结论:化疗、放疗后小鼠乳腺癌的顿抑癌细胞可能是癌局部复发的潜在危险因素。
OBJECTIVE: To investigate the ultrastructural characteristics of tumor suppressor cells survived by chemotherapy and radiotherapy in mouse breast cancer. Methods: Twenty-six breast cancer mice were randomly divided into control group, chemotherapy group (cyclophosphamide, 5-fluorouracil and methotrexate) and radiotherapy group (X-ray). Electron microscopy and computerized image analysis Of surviving cancer cells for morphological analysis. Results: Most of the cancer cells in the cancer tissue after effective chemotherapy and radiotherapy had died, and the well-preserved cancer cells remained in the necrotic cancer tissue. Structural analysis of these cells is still alive, named as Dayton suppress cancer cells, and divided into two categories: type Ⅰ Dayton suppressor cells and type Ⅱ Dayton inhibit cancer cells. The mean volume of tumor suppressor cells was significantly smaller than that of the control group (P <0.001). The body densities of mitochondria and rough endoplasmic reticulum were also significantly lower than those of the control group (P <0.001 or P <0.01) The control group also found the corresponding copy of the corresponding cells. Conclusion: Suppression of cancer cells in breast cancer of mice after chemotherapy and radiotherapy may be potential risk factors of local recurrence of cancer.