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目的建立测定人血浆中伏立康唑浓度的固相萃取-HPLC法。方法用Supelclean LC-18 SPE(500 mg)固相萃取小柱提取人血浆中伏立康唑。色谱柱:Shim-Pack C18(4.6 mm×250 mm,5μm);流动相:甲醇-水(57∶43);流速:1.0 m L·min~(-1);进样量:50μL;检测波长:255 nm;柱温:32℃。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性。结果伏立康唑在0.5~20.0μg·m L~(-1)内线性关系良好,最低定量下限为0.5μg·m L~(-1)(S/N≥3)。伏立康唑的平均提取回收率为88.87%,平均方法回收率为97.17%,平均日内RSD为3.57%,平均日间RSD为4.05%。结论本方法准确、快速、简便,适用于伏立康唑的血浆浓度测定及药代动力学研究。
Objective To establish a solid phase extraction-HPLC method for the determination of voriconazole in human plasma. Methods Voriconazole was extracted from human plasma using a Supelclean LC-18 SPE (500 mg) solid-phase extraction cartridge. Column: Shim-Pack C18 (4.6 mm × 250 mm, 5 μm); mobile phase: methanol-water (57:43); flow rate: 1.0 m L · min -1; : 255 nm; column temperature: 32 ° C. Investigate the specificity of the method, the standard curve and lower limit of quantification, precision and recovery, stability. Results Voriconazole showed a good linearity within 0.5-20.0 μg · m L -1 with the lowest limit of quantitation of 0.5 μg · m L -1 (S / N≥3). The average recovery of voriconazole was 88.87%, the average recovery rate was 97.17%, the average daily RSD was 3.57% and the average daytime RSD was 4.05%. Conclusion The method is accurate, rapid and simple and is suitable for the determination of voriconazole in plasma and its pharmacokinetics.