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本文报道运用Percoll 梯度离心技术,分离纯化大鼠附睾头、体、尾各段精子(20例),再用硫代巴比妥酸法对精子膜唾液酸含量依次进行测定,结果显示:大鼠附睾头、体、尾各段精子膜唾液酸含量具有显著差异(P<0.01),各段精子膜唾液酸量分别为10.18±2.82、8.42±3.077、83±2.79μg/10~8精子(X±SD)。用胶体铁组化染色也证实精子在附睾内转运时膜唾液酸量呈下降趋势。由此提示:精子膜唾液酸含酸量与精子在附睾内成熟即受精能力的发育与维持有十分密切的关系。
This paper reports the use of Percoll gradient centrifugation technique to separate and purify the spermatozoa in the head, tail and body of the rat (20 cases), and then determine the content of sialic acid in the sperm membrane by thiobarbituric acid method. The results show that: The contents of sialic acid in the spermatozoa of the epididymis head, body and tail were significantly different (P <0.01). The sialic acid contents of each sperm membrane were 10.18 ± 2.82, 8.42 ± 3.077 and 83 ± 2.79μg / 10 ~ 8 respectively ± SD). Colloidal iron staining also confirmed sperm transit in the epididymis membrane sialic acid was a downward trend. It is suggested that the sperm acid content of sperm membrane and sperm in the epididymis in the development of fertilization that is able to maintain and maintain a very close relationship.