基于Sestrin2通过mTOR信号通路调节胰腺癌上皮-间质转化过程的实验研究

来源 :肝胆胰外科杂志 | 被引量 : 0次 | 上传用户：Air8712

【摘要】

：

目的探讨Sestrin 2在胰腺癌上皮-间质转化(EMT)过程中的作用。方法对胰腺癌细胞PANC-1和CFPAC-1进行Sestrin 2表达干预,通过划痕试验、Transwell试验、RT-PCR、Western blott

【作者】

：

戴胜杰陈豪楼彬孙洪伟

【机构】

：

温州医科大学附属第一医院肝胆胰外科,浙江省杭州市余杭区第三人民医院普外科

【出处】

：

肝胆胰外科杂志

【发表日期】

：

2021年6期

【关键词】

：

胰腺癌上皮-间质转化(EMT) Sestrin2 mTOR

【基金项目】

：

浙江省自然科学基金探索项目Q(LQ20H160014),浙江省卫生厅卫生医药计划(2019RC210),浙江省自然科学基金探索项目Y(LY20H160012)。

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目的探讨Sestrin 2在胰腺癌上皮-间质转化(EMT)过程中的作用。方法对胰腺癌细胞PANC-1和CFPAC-1进行Sestrin 2表达干预,通过划痕试验、Transwell试验、RT-PCR、Western blotting和动物实验检测不同Sestrin 2表达水平下胰腺癌EMT过程的变化。同时,分析Sestrin 2在人体中的表达情况。结果在Transwell试验和划痕试验中,高表达Sestrin 2胰腺癌细胞组的迁移能力和侵袭能力显著降低(P<0.05);在动物负瘤实验中,高表达Ses

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基于Sestrin2通过mTOR信号通路调节胰腺癌上皮-间质转化过程的实验研究

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