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利用一套陆地棉RIL群体QTL定位的结果,结合随机抽取的第116,118株系DNA重组片段来源以及它们田间性状对QTLs的关联分析,基于QTG层面探讨了纯合DNA因子对杂种优势的贡献,试图总结出“显性+超显性+上位性”的观点,即加性及加性上位性是杂种优势的遗传基础,而显性、超显性、上位性仅为杂种优势的作用方式.结合本研究检测到的杂种优势,简要总结了杂种优势的分子机理,并以该2个株系为例进行详解:在连锁群LG01和LG03上的上半部平均长度分别来自母本、父本的加性上位性QTLs,对应于2位点均来自母本的加性上位性QTLs可产生2.99~3.52的差异.单株铃数受2对加性上位性QTLs控制,存在于LG02和LG07上正反互作的加性上位性QTLs可产生0.86的差异.依据加性效应遗传特点可初步提出构建棉花“超级杂交种”的设想.
Based on the results of QTL mapping of a RIL population from a set of upland cotton, combined with the source of DNA recombination segments of 116,118 randomly selected strains and their correlation analysis of QTLs in field traits, the contribution of homozygous DNA factors to heterosis was discussed based on QTG level. Summed up the “dominant + super dominant + epistasis ” point of view, that is additive and additive epistasis is the genetic basis of heterosis, and dominance, super dominant, epistasis is only the role of heterosis Combining with the heterosis detected in this study, the molecular mechanism of heterosis was briefly summarized and the two strains were taken as an example to explain in detail: the average length of the upper half of the linkage groups LG01 and LG03 were respectively from the female parent and father The additive epistatic QTLs of this locus, which corresponded to the additive epistatic QTLs from the female parent at 2 loci, could produce a difference of 2.99-3.52. The number of bolls per plant was controlled by 2 pairs of additive epistatic QTLs and existed in LG02 and LG07 The positive and negative additive epistatic QTLs can produce a difference of 0.86.According to the genetic characteristics of additive effects, we can tentatively put forward the idea of constructing “super hybrid” cotton.