论文部分内容阅读
为深入探讨拟除虫菊酯和有机磷混配农药对中枢神经系统的毒性作用及其机制,通过免疫组织化学和显微图象分析方法观察了大鼠经氰戊菊酯(20m g/kgBW)、辛硫磷(160m g/kgBW)、氰戊菊酯+ 辛硫磷(180m g/kgBW,1v8等毒配比)灌胃染毒24小时后中枢神经组织中谷氨酸(Glu)和γ- 氨基丁酸(GABA)免疫阳性细胞(IRC)的变化。结果显示,氰戊菊酯组、氰戊菊酯+ 辛硫磷组大鼠大脑皮层、海马、纹状体等部位Glu的IRC数目(N)减少,阳性细胞面积比(Aa% )和积分光密度(IOD)呈现不同程度的降低,而GABA的IRC各观察参数值均呈现不同程度的升高;氰戊菊酯组与氰戊菊酯+ 辛硫磷组各参数值之间未见显著性差异;与对照组相比,单独辛硫磷组各观察参数值亦未见显著性变化。本次研究结果提示,Glu、GABA递质功能紊乱可能在拟除虫菊酯神经毒性中具有重要意义;在对Glu 和GABA作用的影响上,拟除虫菊酯和有机磷之间无协同作用
In order to further explore the toxic effects of pyrethroids and organophosphorus pesticides on the central nervous system and its mechanism, the effects of fenvalerate (20 m g / kg BW) Phosphatidylcholine (160 m g / kg BW) and fenvalerate + phoxim (180 m g / kg BW, 1 v8) were intragastrically administered for 24 hours after glutamate (Glu) and γ- Changes of butyric acid (GABA) immunoreactive cells (IRC). The results showed that the IRC number (N) of Glu decreased in the fenvalerate group and the fenvalerate + phoxim group, and the percentage of positive cell area (Aa%) and integral light Density (IOD) showed varying degrees of decline, and GABA IRC observed values ?? of all showed varying degrees of increase; fenvalerate group and fenvalerate + phoxim group of parameters between the values were not significant Differences; compared with the control group, the individual phoxim group observed values ?? also no significant change. The results of this study suggest that Glu and GABA neurotransmitter dysfunction may be of great importance in pyrethroid neurotoxicity; there is no synergistic effect between pyrethroids and organophosphorus on the effects of Glu and GABA