论文部分内容阅读
目的:探讨UPA,PAI-1在结直肠癌血浆中的浓度变化,及其作为肿瘤指标的临床应用价值。方法:使用ELISA方法测定20名健康人群血浆UPA,PAI-1浓度,以及48例结直肠癌患者治疗前血浆UPA,PAI-1浓度(其中22例追踪测定术后血样浓度)。同时期测定血清CEA浓度。结果:UPA、PAI-1在48例结直肠癌患者血浆中的浓度明显高于对照组(P<0.05)。追踪22例患者根治术前后血浆UPA、PAI-1浓度变化,其浓度显著下降。血浆UPA在DukesC、D期,以及转移的患者中浓度较高。而PAI-1在DukesD期患者中明显升高。血浆UPA,PAI-1浓度有微弱的相关(r=0.523)。在早期诊断中,UPA、PAI-1的敏感性分别为78.17%、39.58%。特异度分别为90%、95%。多个指标联合检测时敏感度明显上升,其中UPA和CEA联合检测效果最佳,敏感度83.33%。结论:UPA、PAI-1在结直肠肿瘤进展中起重要作用。作为早期诊断指标,UPA好于CEA和PAI-1。联合检测UPA与CEA,准确性更好。
Objective: To investigate the changes of plasma concentrations of UPA and PAI-1 in colorectal cancer and their clinical value as tumor markers. Methods: Plasma concentrations of UPA and PAI-1 were measured in 20 healthy subjects and plasma UPA and PAI-1 concentration in 48 patients with colorectal cancer before and after treatment (of which 22 were followed up for blood concentration measurement). The same period measured serum CEA concentration. Results: The plasma levels of UPA and PAI-1 in 48 patients with colorectal cancer were significantly higher than those in the control group (P <0.05). The changes of plasma concentrations of UPA and PAI-1 in 22 patients before and after radical surgery were observed, and their concentrations were significantly decreased. Plasma UPA is highly concentrated in DukesC, D, and metastatic patients. PAI-1 was significantly elevated in patients with DukesD stage. There was a weak correlation between plasma UPA and PAI-1 concentrations (r = 0.523). In early diagnosis, the sensitivity of UPA and PAI-1 were 78.17% and 39.58% respectively. Specificity was 90%, 95%. Sensitivity of multiple indicators increased significantly when combined detection, of which UPA and CEA combined detection the best effect, the sensitivity of 83.33%. Conclusion: UPA and PAI-1 play an important role in the progression of colorectal cancer. As an early diagnostic indicator, UPA is better than CEA and PAI-1. Joint detection of UPA and CEA, accuracy is better.