Paddy Husk as Support for Solid State Fermentation to Produce Xylanase from Bacillus pumilus

来源 :Rice Science | 被引量 : 0次 | 上传用户:Ivy1234
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
To optimize culture conditions for xylanase production by solid state fermentation (SSF) using Bacillus pumilus, with paddy husk as support, solid medium contained 200 g of paddy husk with 800 mL of liquid fermentation medium [xylan, 20.0 g/L; peptone, 2.0 g/L; yeast extract, 2.5 g/L; K2HPO4 , 2.5 g/L; KH2PO4 , 1.0 g/L; NaCl, 0.1 g/L; (NH4)2SO4 , 2.0 g/L, CaCl2 ·2H2O, 0.005 g/L; MgCl2 ·6H2O, 0.005 g/L; and FeCl 3 , 0.005 g/L] at pH 9.0 was applied. The highest xylanase activity (142.0 ±0.47 U/g DM] was obtained on the 6 th day at 30 ℃. The optimized paddy husk to liquid fermentation medium ratio was 2:9, and the optimized culture temperature was 40℃. When commercial Birchwood xylan was replaced with different concentrations of corncob, xylanase production was maximized (224.2 U/g DM) in the medium with 150 g/L corncob. Xylanase production was increased by sucrose, fructose and arabinose, whereas reduced by glucose, galactose, lactose and amylose. When organic nitrogen sources were replaced with locally available nitrogen sources such as groundnut powder or sesame seedcake powder or coconut seedcake powder or soy meal powder, the highest xylanase production (290.7 U/g DM) was obtained in the medium with soy meal powder and 16.0 g/L of soy meal powder was the optimum (326.5±0.34 U/g DM). Based on the optimization studies, B. pumilus produced 2.3 times higher xylanase activity. The medium cost was reduced from 2 458.3 to 178.3 SLR/kg and the total activity which could be obtained from 1 kg of the medium was increased from 48 624 to 220 253 Units. To optimize culture conditions for xylanase production by solid state fermentation (SSF) using Bacillus pumilus, with paddy husk as support, solid medium containing 200 g of paddy husk with 800 mL of liquid fermentation medium [xylan, 20.0 g / L; peptone, 2.0 (g / L), yeast extract 2.5 g / L, K2HPO4 2.5 g / L, KH2PO4 1.0 g / L, NaCl 0.1 g / L, NH4SO4 2.0 g / L, CaCl2 2H2O 0.005 g / L; MgCl2.6H2O, 0.005 g / L; and FeCl3, 0.005 g / L] at pH 9.0 was applied. The highest xylanase activity (142.0 ± 0.47 U / g DM] was obtained on the 6th day at 30 ° C. When the commercial Birchwood xylan was replaced with different concentrations of corncob, xylanase production was maximized (224.2 U / g DM) in the medium with 150 g / L corncob. Xylanase production was increased by sucrose, fructose and arabinose, reduced reduced by glucose, galactose, lactose and amylose. When organic nitrogen sources we re replaced with locally available nitrogen sources such as groundnut powder or sesame seedcake powder or coconut seedcake powder or soy meal powder, the highest xylanase production (290.7 U / g DM) was obtained in the medium with soy meal powder and 16.0 g / L of Based on the optimization studies, B. pumilus produced 2.3 times higher xylanase activity. The medium cost was reduced from 2 458.3 to 178.3 SLR / kg and the total activity which could have obtained from 1 kg of the medium was increased from 48 624 to 220 253 Units.
其他文献
依据菏泽市连续运行卫星定位服务系统建设的资料,分析了HZCORS数据中心的主要功能和设计特点,论述了安全性、稳定性在HZCORS中的重要性.
隐喻过去仅被人们视为一种修辞手段.但认知语言学家发现,它已经超越了修辞的限制,成为一种普遍的认知方式,即概念隐喻.它不仅存在于我们的语言中,同时还存在于我们的思想和行
新世纪职专语文教学中的人文精神渗透,追求阅读的社会化、民族化、人格化、个性化,使阅读课堂不仅成为学生获得阅读知识、习得阅读能力的场所,而且成为学生开发智力、体验人
学生已经意识到通过实习的方式,不仅可以积累企业的工作经验,还能消除与企业之间的沟通障碍。新生专业实习,是学生认识行业性质、培养动手能力的有效途径。然而,在实习过程中
是志怪小说走向成熟的开山之作,它具有圆融混合的结构和离奇怪异的情节,因而成为唐传奇艺术史上的奇葩.从叙事策略的角度去考察贯穿各自独立的单篇故事所运用的手法,分析故事
《简.爱》是英国文学史上一部具有代表性的经典名著,多年来深受读者的喜爱。《简.爱》在中国有多种译本,其中以李霁野的译本为最早与祝庆英的译本最广为流传而深入人心。由于
金矿的成矿因素复杂而多样,在众多的影响因素中构造、应力、热液运行是使之形成聚集而沉积的重要条件,而在实际的找矿作业中还需要对地层压性、断裂构造、化学成分等进行细致的
随着互联网的迅猛发展和普及应用,基于互联网的会计信息系统已成为必然的发展趋势,本文从COSO框架确立的财务控制五要素出发,对IT环境下的财务制度存在的问题及风险进行深入
We have analyzed the July 15,2009,Fiordland earthquake(M_w 7.8),which occurred in the southwestern part of the South Island of New Zealand.This thrust-related earthquake in the southern Fiordland subd
现代测绘技术是运用到地籍测量中的一些先进的技术和方法,它是融地籍测量外业、内业于一体的综合性作业系统.文章对测绘技术在地籍测量中的应用进行了介绍.