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目的:观察S100A8、S100A9蛋白在比格犬健康及实验性牙周炎组织中的表达分布特点。方法:将6只比格犬的一侧下颌第二磨牙通过拴线法诱导实验性牙周炎模型（结扎组），另一侧下颌第二磨牙保持口腔卫生（健康对照组），应用免疫组化法检测S100A8、S100A9在6只比格犬健康及实验性牙周炎组织中的表达；免疫细胞化学法检测两种蛋白亚基在人牙龈成纤维细胞（human gingival fibroblasts，hGF）（来自3例牙冠延长术切除的牙龈组织）、人牙周膜细胞（human periodontal ligament cells，hPDLC）（来自3例因正畸拔除的前磨牙或第三磨牙的牙周膜细胞）中的表达。结果:实验性牙周炎诱导第12周，结扎组探诊深度[（3.86±0.14）mm]显著高于健康对照组[（2.11±0.28）mm，n P<0.01]；比格犬健康牙周组织中S100A8、S100A9主要表达于牙龈上皮细胞、中性粒细胞，且在结合上皮处呈强阳性表达；实验性牙周炎组织中除牙龈上皮、中性粒细胞外，两种蛋白还诱导表达于牙龈成纤维细胞、牙周膜细胞、微血管内皮细胞及骨髓成纤维细胞；hGF及hPDLC中均可检测到S100A8、S100A9的表达。n 结论:实验性牙周炎使S100A8、S100A9的表达范围更大，表达S100A8、S100A9的细胞类型更多。“,”Objective:To investigate the systemic expression profile of S100A8 and S100A9 in healthy and inflamed periodontal tissues.Methods:Experimental periodontitis models were established by ligations around the mandibular second molars of six beagle dogs for 12 weeks (ligation group). The mandibular second molars on the opposite side were kept clean (healthy control group). The expressions of S100A8 and S100A9 in healthy and inflamed periodontal tissues of six beagle dogs were examined by immunohistochemistry. The expressions of S100A8 and S100A9 in primary human gingival fibroblasts (hGF) from 3 subjects and human periodontal ligament cells (hPDLC) from 3 other subjects were detected by immunocytochemistry.Results:After the ligation for 12 weeks, the mean probing depth of ligation group [(3.86±0.14) mm] was significantly higher than that of healthy control group [(2.11±0.28) mm] (n P<0.01). Results of immunohistochemistry analysis indicated that S100A8 and S100A9 could be expressed in gingival epithelial cells and might infiltrated neutrophils in the healthy periodontium. Except for the gingival epithelial cells and neutrophils, both proteins were induced and expressed in gingival fibroblasts, periodontal ligament cells, microvascular endothelial cells and bone marrow fibroblasts under inflammatory conditions. The distribution of S100A8 and S100A9 differed in the healthy oral gingival epithelium (OGE), which becomes consistent in inflamed OGE. Additionally, the expressions of S100A8 and S100A9 were confirmed in primary hGF and hPDLC.n Conclusions:Periodontal inflammation might enlarge the expression scope of S100A8 and S100A9 and enrich multiple cells with expressions of S100A8 and S100A9.