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目的观察三黄泻心汤“炮制配伍”解热优势方药效并初步探究其作用机制。方法大鼠皮下注射20%干酵母混悬液复制发热模型,阿司匹林(0.1g·kg~(-1))作为阳性对照药,三黄泻心汤解热优势方分为3个剂量(1、0.5、0.25 g生药·kg~(-1)),灌胃给药。首先测量给药前基础体温,然后分别于给药后0.5、1、2、4 h测量大鼠肛温,以实测体温-基础体温(ΔT)表示体温变化,实验结束后采血及分离血清,取下丘脑组织后制备上清。Elisa法测定血清和下丘脑组织中PGE2、c AMP、Cox-1、Cox-2的含量。结果三黄泻心汤“炮制配伍”解热优势方各剂量组均可使体温下降,并降低发热大鼠血清和下丘脑组织中PGE2、cAMP、Cox-1、Cox-2的含量。结论三黄泻心汤“炮制配伍”解热优势方有明显的解热作用,其机制可能是与抑制PGE_2、c AMP等中枢发热介质的释放有关。
Objective To observe the efficacy of “Sanhuang Xiexin Decoction” and “Concocted compatibility” antipyretic prescription and to explore its mechanism. Methods The model of fever was replicated by subcutaneous injection of 20% dry yeast suspension. Aspirin (0.1g · kg -1) was used as a positive control. The antipyretic prescription of Sanhuang Xiexin Decoction was divided into three doses (1, 0.5,0.25 g crude drug · kg -1), administered orally. The basal body temperature before administration was measured, and the rectal temperature was measured at 0.5, 1, 2 and 4 h after administration, respectively. The body temperature and basal body temperature (ΔT) were measured to indicate the change of body temperature. After the experiment, serum was collected and separated Hypothalamic tissue is prepared after supernatant. The contents of PGE2, cAMP, Cox-1 and Cox-2 in serum and hypothalamus were measured by Elisa method. ResultsShuanghuangxiexin Decoction “Concocted compatibility” antipyretic side effects of each dose group can reduce body temperature and reduce the content of PGE2, cAMP, Cox-1 and Cox-2 in the serum and hypothalamus of rats with fever. Conclusions Sanhuangxiexin Decoction “Concocted compatibility” has obvious antipyretic effect, and its mechanism may be related to the inhibition of the release of PGE 2, c AMP and other central heating medium.