对活体松材线虫样品进行处理 ,用透射电镜对松材线虫各部分进行切片和详细观察 ,未能在线虫体内的任何部位观察到细菌。然而 ,用较少脱水步骤的扫描电镜样品处理方法处理活体松材线虫 ,用扫描电镜在线虫体表观察到了许多细菌 ,这些细菌形态一致、杆状、长 1 .48～1 .73μm、直径 0 .49～ 0 .62 μm。本文是关于松材线虫携带细菌部位的首次报道 The samples of live pine wood nematode were treated, and the sections of pine wood nematode were sectioned and observed with TEM. The bacteria were not observed in any part of the nematode. However, scanning electron microscopy with less dehydration steps was used to treat live pine wood nematodes, and many bacteria were observed on the nematodes surface by scanning electron microscopy. The bacteria were morphologically uniform and rod-shaped with a length of 1.48-1.73 μm and a diameter of 0 .49 ~ 0.62 μm. This article is the first to report that pine wood nematodes carry bacterial sites